Next Article in Journal
Peroxisomes and Innate Immunity: Antiviral Response and Beyond
Previous Article in Journal
Testing Anti-Biofilm Polymeric Surfaces: Where to Start?
Open AccessArticle

GmFAD3A, A ω-3 Fatty Acid Desaturase Gene, Enhances Cold Tolerance and Seed Germination Rate under Low Temperature in Rice

1
Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang 330031, China
2
National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
3
Key Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
4
Jiangxi Super-Rice Research and Development Center, Nanchang 330200, China
5
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(15), 3796; https://doi.org/10.3390/ijms20153796
Received: 19 June 2019 / Revised: 27 July 2019 / Accepted: 30 July 2019 / Published: 3 August 2019
(This article belongs to the Section Molecular Plant Sciences)
Low temperature is an environmental stress factor that is always been applied in research on improving crop growth, productivity, and quality of crops. Polyunsaturated fatty acids (PUFAs) play an important role in cold tolerance, so its genetic manipulation of the PUFA contents in crops has led to the modification of cold sensitivity. In this study, we over-expressed an ω-3 fatty acid desaturase from Glycine max (GmFAD3A) drove by a maize ubiquitin promoter in rice. Compared to the wild type (ZH11), ectopic expression of GmFAD3A increased the contents of lipids and total PUFAs. Seed germination rates in GmFAD3A transgenic rice were enhanced under low temperature (15 °C). Moreover, cold tolerance and survival ratio were significantly improved in GmFAD3A transgenic seedlings. Malondialdehyde (MDA) content in GmFAD3A transgenic rice was lower than that in WT under cold stress, while proline content obviously increased. Meanwhile, the activities of superoxide dismutase (SOD), hydroperoxidase (CAT), and peroxidase (POD) increased substantially in GmFAD3A transgenic rice after 4 h of cold treatment. Taken together, our results suggest that GmFAD3A can enhances cold tolerance and the seed germination rate at a low temperature in rice through the accumulation of proline content, the synergistic increase of the antioxidant enzymes activity, which finally ameliorated the oxidative damage. View Full-Text
Keywords: Oryza sativa; ω-3 fatty acid desaturase; GmFAD3A; cold stress; seed germination Oryza sativa; ω-3 fatty acid desaturase; GmFAD3A; cold stress; seed germination
Show Figures

Figure 1

MDPI and ACS Style

Wang, X.; Yu, C.; Liu, Y.; Yang, L.; Li, Y.; Yao, W.; Cai, Y.; Yan, X.; Li, S.; Cai, Y.; Li, S.; Peng, X. GmFAD3A, A ω-3 Fatty Acid Desaturase Gene, Enhances Cold Tolerance and Seed Germination Rate under Low Temperature in Rice. Int. J. Mol. Sci. 2019, 20, 3796.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop