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Radiosensitivity Differences between EGFR Mutant and Wild-Type Lung Cancer Cells are Larger at Lower Doses

1
Department of Radiation Oncology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan
2
Division of Radiation Oncology, Department of Radiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
3
Gunma University Initiative for Advanced Research (GIAR), Gunma University, Maebashi 371-8511, Japan
*
Author to whom correspondence should be addressed.
These authors equally contributed.
Int. J. Mol. Sci. 2019, 20(15), 3635; https://doi.org/10.3390/ijms20153635
Received: 27 June 2019 / Revised: 18 July 2019 / Accepted: 23 July 2019 / Published: 25 July 2019
(This article belongs to the Special Issue Counteracting Radioresistance Using the Optimization of Radiotherapy)
In the era of precision medicine, radiotherapy strategies should be determined based on genetic profiles that predict tumor radiosensitivity. Accordingly, pre-clinical research aimed at discovering clinically applicable genetic profiles is needed. However, how a given genetic profile affects cancer cell radiosensitivity is unclear. To address this issue, we performed a pilot in vitro study by utilizing EGFR mutational status as a model for genetic profile. Clonogenic assays of EGFR mutant (n = 6) and wild-type (n = 9) non-small cell lung carcinoma (NSCLC) cell lines were performed independently by two oncologists. Clonogenic survival parameters SF2, SF4, SF6, SF8, mean inactivation dose (MID), D10, D50, α, and β were obtained using the linear quadratic model. The differences in the clonogenic survival parameters between the EGFR mutant and wild-type cell lines were assessed using the Mann–Whitney U test. As a result, for both datasets, the p values for SF2, SF4, D50, α, and α/β were below 0.05, and those for SF2 were lowest. These data indicate that a genetic profile of NSCLC cell lines might be predictive for their radiation response; i.e., EGFR mutant cell lines might be more sensitive to low dose- and low fraction sized-irradiation. View Full-Text
Keywords: precision medicine; radiation therapy; radiosensitivity; clonogenic assays; gene mutations precision medicine; radiation therapy; radiosensitivity; clonogenic assays; gene mutations
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MDPI and ACS Style

Anakura, M.; Nachankar, A.; Kobayashi, D.; Amornwichet, N.; Hirota, Y.; Shibata, A.; Oike, T.; Nakano, T. Radiosensitivity Differences between EGFR Mutant and Wild-Type Lung Cancer Cells are Larger at Lower Doses. Int. J. Mol. Sci. 2019, 20, 3635. https://doi.org/10.3390/ijms20153635

AMA Style

Anakura M, Nachankar A, Kobayashi D, Amornwichet N, Hirota Y, Shibata A, Oike T, Nakano T. Radiosensitivity Differences between EGFR Mutant and Wild-Type Lung Cancer Cells are Larger at Lower Doses. International Journal of Molecular Sciences. 2019; 20(15):3635. https://doi.org/10.3390/ijms20153635

Chicago/Turabian Style

Anakura, Mai; Nachankar, Ankita; Kobayashi, Daijiro; Amornwichet, Napapat; Hirota, Yuka; Shibata, Atsushi; Oike, Takahiro; Nakano, Takashi. 2019. "Radiosensitivity Differences between EGFR Mutant and Wild-Type Lung Cancer Cells are Larger at Lower Doses" Int. J. Mol. Sci. 20, no. 15: 3635. https://doi.org/10.3390/ijms20153635

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