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Open AccessArticle

Overexpression of Notch Signaling Induces Hyperosteogeny in Zebrafish

1
Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
2
Department of Biological Science & Technology College of Medicine, I-Shou University, Kaohsiung 84001, Taiwan
3
Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan
4
Center for Biomedical Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
5
Center for Nanotechnology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(15), 3613; https://doi.org/10.3390/ijms20153613
Received: 11 May 2019 / Revised: 3 July 2019 / Accepted: 18 July 2019 / Published: 24 July 2019
(This article belongs to the Special Issue Bone Growth and Osteoporosis)
Notch signaling is one of the evolutionarily conserved signaling pathways in multicellular organisms. It plays an important role in embryonic development. During skeletal development of vertebrates, it regulates bone homeostasis by manipulating both osteoblastogenesis and osteoclastogenesis through different mechanisms. However, due to the different nature of Notch signaling in mesenchymal stem cell and osteoblast, regulation of Notch signaling in bone-related diseases remains unsettled. Previous studies by cell culture and mouse models showed contradictory results regarding the role of Notch signaling in bone homeostasis. To clarify the role of Notch signaling in osteogenesis, we established a zebrafish model, in which Notch1a intracellular domain (N1aICD) was specifically expressed in the osteoblasts. We found that overexpression of N1aICD in osteoblasts caused hyperosteogeny in the column region of zebrafish with the morphology of narrowed neural/hemal canals. Moreover, increased metabolic activity of osteoblasts instead of augmenting osteoblast number led to hyperosteogeny in N1aICD-overexpressed zebrafish. In summary, we successfully established a transgenic zebrafish line overexpressing N1aICD to clarify the in-vivo function of Notch signaling during osteoblastogenesis. In the future, this fish line can serve as a valuable tool to test the therapeutic drugs for hyperosteogeny. View Full-Text
Keywords: bone; hyperosteogeny; notch; transgenic zebrafish bone; hyperosteogeny; notch; transgenic zebrafish
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Liang, S.-T.; Chen, J.-R.; Tsai, J.-J.; Lai, Y.-H.; Hsiao, C.-D. Overexpression of Notch Signaling Induces Hyperosteogeny in Zebrafish. Int. J. Mol. Sci. 2019, 20, 3613.

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