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Open AccessArticle

Deciphering the Origin and Evolution of the X1X2Y System in Two Closely-Related Oplegnathus Species (Oplegnathidae and Centrarchiformes)

1
Key Lab of Mariculture and Enhancement of Zhejiang Province, Marine Fishery Institute of Zhejiang Province, Zhoushan 316100, China
2
College of Fisheries, Zhejiang Ocean University, Zhoushan 316100, China
3
Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Rumburská 89, 277 21 Liběchov, Czech Republic
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Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), Rodovia Washington Luiz Km. 235, C.P. 676, São Carlos SP 13565-905, Brazil
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Secretaria de Estado de Educação de Mato Grosso—SEDUC-MT, Cuiabá MT 78049-909, Brazil
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University Clinic Jena, Institute of Human Genetics, 07747 Jena, Germany
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Laboratory for Marine Biology and Biotechnology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(14), 3571; https://doi.org/10.3390/ijms20143571
Received: 12 June 2019 / Revised: 5 July 2019 / Accepted: 13 July 2019 / Published: 22 July 2019
(This article belongs to the Special Issue Chromosome and Karyotype Variation)
Oplegnathus fasciatus and O. punctatus (Teleostei: Centrarchiformes: Oplegnathidae), are commercially important rocky reef fishes, endemic to East Asia. Both species present an X1X2Y sex chromosome system. Here, we investigated the evolutionary forces behind the origin and differentiation of these sex chromosomes, with the aim to elucidate whether they had a single or convergent origin. To achieve this, conventional and molecular cytogenetic protocols, involving the mapping of repetitive DNA markers, comparative genomic hybridization (CGH), and whole chromosome painting (WCP) were applied. Both species presented similar 2n, karyotype structure and hybridization patterns of repetitive DNA classes. 5S rDNA loci, besides being placed on the autosomal pair 22, resided in the terminal region of the long arms of both X1 chromosomes in females, and on the X1 and Y chromosomes in males. Furthermore, WCP experiments with a probe derived from the Y chromosome of O. fasciatus (OFAS-Y) entirely painted the X1 and X2 chromosomes in females and the X1, X2, and Y chromosomes in males of both species. CGH failed to reveal any sign of sequence differentiation on the Y chromosome in both species, thereby suggesting the shared early stage of neo-Y chromosome differentiation. Altogether, the present findings confirmed the origin of the X1X2Y sex chromosomes via Y-autosome centric fusion and strongly suggested their common origin. View Full-Text
Keywords: comparative genomic hybridization; centric fusion; multiple sex chromosomes; Oplegnathus; whole chromosome painting comparative genomic hybridization; centric fusion; multiple sex chromosomes; Oplegnathus; whole chromosome painting
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Xu, D.; Sember, A.; Zhu, Q.; Oliveira, E.A.; Liehr, T.; Al-Rikabi, A.B.H.; Xiao, Z.; Song, H.; Cioffi, M.B. Deciphering the Origin and Evolution of the X1X2Y System in Two Closely-Related Oplegnathus Species (Oplegnathidae and Centrarchiformes). Int. J. Mol. Sci. 2019, 20, 3571.

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