Next Article in Journal
14-3-3 Proteins Are on the Crossroads of Cancer, Aging, and Age-Related Neurodegenerative Disease
Next Article in Special Issue
Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression
Previous Article in Journal
Prioritization of Variants for Investigation of Genotype-Directed Nutrition in Human Superpopulations
Previous Article in Special Issue
Auraptene Mitigates Parkinson’s Disease-Like Behavior by Protecting Inhibition of Mitochondrial Respiration and Scavenging Reactive Oxygen Species
Open AccessArticle

Flavonoids from Chionanthus retusus (Oleaceae) Flowers and Their Protective Effects against Glutamate-Induced Cell Toxicity in HT22 Cells

1
Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University, Yongin 17104, Korea
2
College of Pharmacy, Chosun University, Gwangju 61452, Korea
3
Strategic Planning Division, National Institute of Biological Resources, Incheon 22689, Korea
4
Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(14), 3517; https://doi.org/10.3390/ijms20143517
Received: 31 May 2019 / Revised: 13 July 2019 / Accepted: 15 July 2019 / Published: 18 July 2019
(This article belongs to the Special Issue Natural Products and Neuroprotection)
  |  
PDF [1958 KB, uploaded 18 July 2019]
  |     |  

Abstract

The dried flowers of Chionanthus retusus were extracted with 80% MeOH, and the concentrate was divided into EtOAc, n-BuOH, and H2O fractions. Repeated SiO2, octadecyl SiO2 (ODS), and Sephadex LH-20 column chromatography of the EtOAc fraction led to the isolation of four flavonols (14), three flavones (57), four flavanonols (811), and one flavanone (12), which were identified based on extensive analysis of various spectroscopic data. Flavonoids 46 and 811 were isolated from the flowers of C. retusus for the first time in this study. Flavonoids 1, 2, 5, 6, 8, and 1012 significantly inhibited NO production in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) and glutamate-induced cell toxicity and effectively increased HO-1 protein expression in mouse hippocampal HT22 cells. Flavonoids with significant neuroprotective activity were also found to recover oxidative-stress-induced cell damage by increasing HO-1 protein expression. This article demonstrates that flavonoids from C. retusus flowers have significant potential as therapeutic materials in inflammation and neurodisease. View Full-Text
Keywords: Chionanthus retusus; flavonoid; flower; HO-1; neuroprotection; NO Chionanthus retusus; flavonoid; flower; HO-1; neuroprotection; NO
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Lee, Y.-G.; Lee, H.; Jung, J.-W.; Seo, K.-H.; Lee, D.Y.; Kim, H.-G.; Ko, J.-H.; Lee, D.-S.; Baek, N.-I. Flavonoids from Chionanthus retusus (Oleaceae) Flowers and Their Protective Effects against Glutamate-Induced Cell Toxicity in HT22 Cells. Int. J. Mol. Sci. 2019, 20, 3517.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top