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Article

Magnesium Deficiency Induced Global Transcriptome Change in Citrus sinensis Leaves Revealed by RNA-Seq

1
Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(13), 3129; https://doi.org/10.3390/ijms20133129
Received: 9 June 2019 / Revised: 21 June 2019 / Accepted: 25 June 2019 / Published: 26 June 2019
(This article belongs to the Section Molecular Plant Sciences)
Magnesium (Mg) deficiency is one of the major constraining factors that limit the yield and quality of agricultural products. Uniform seedlings of the Citrus sinensis were irrigated with Mg deficient (0 mM MgSO4) and Mg sufficient (1 mM MgSO4) nutrient solutions for 16 weeks. CO2 assimilation, starch, soluble carbohydrates, TBARS content and H2O2 production were measured. Transcriptomic analysis of C. sinensis leaves was performed by Illumina sequencing. Our results showed that Mg deficiency decreased CO2 assimilation, but increased starch, sucrose, TBARS content and H2O2 production in C. sinensis leaves. A total of 4864 genes showed differential expression in response to Mg deficiency revealed by RNA-Seq and the transcriptomic data were further validated by real-time quantitative PCR (RT-qPCR). Gene ontology (GO) enrichment analysis indicated that the mechanisms underlying Mg deficiency tolerance in C. sinensis may be attributed to the following aspects: (a) enhanced microtubule-based movement and cell cycle regulation; (b) elevated signal transduction in response to biotic and abiotic stimuli; (c) alteration of biological processes by tightly controlling phosphorylation especially protein phosphorylation; (d) down-regulation of light harvesting and photosynthesis due to the accumulation of carbohydrates; (e) up-regulation of cell wall remodeling and antioxidant system. Our results provide a comprehensive insight into the transcriptomic profile of key components involved in the Mg deficiency tolerance in C. sinensis and enrich our understanding of the molecular mechanisms by which plants adapted to a Mg deficient condition. View Full-Text
Keywords: Mg deficiency; Citrus sinensis; transcriptome; cellular transport; signal transduction Mg deficiency; Citrus sinensis; transcriptome; cellular transport; signal transduction
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MDPI and ACS Style

Yang, L.-T.; Zhou, Y.-F.; Wang, Y.-Y.; Wu, Y.-M.; Ye, X.; Guo, J.-X.; Chen, L.-S. Magnesium Deficiency Induced Global Transcriptome Change in Citrus sinensis Leaves Revealed by RNA-Seq. Int. J. Mol. Sci. 2019, 20, 3129. https://doi.org/10.3390/ijms20133129

AMA Style

Yang L-T, Zhou Y-F, Wang Y-Y, Wu Y-M, Ye X, Guo J-X, Chen L-S. Magnesium Deficiency Induced Global Transcriptome Change in Citrus sinensis Leaves Revealed by RNA-Seq. International Journal of Molecular Sciences. 2019; 20(13):3129. https://doi.org/10.3390/ijms20133129

Chicago/Turabian Style

Yang, Lin-Tong, Yang-Fei Zhou, Yan-Yu Wang, Yan-Mei Wu, Xin Ye, Jiu-Xin Guo, and Li-Song Chen. 2019. "Magnesium Deficiency Induced Global Transcriptome Change in Citrus sinensis Leaves Revealed by RNA-Seq" International Journal of Molecular Sciences 20, no. 13: 3129. https://doi.org/10.3390/ijms20133129

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