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Open AccessArticle

Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle

1
National Medical Research Center of Cardiology, Russian Ministry of Health, 121552 Moscow, Russia
2
Faculty of Medicine, Lomonosov Moscow State University, 119991 Moscow, Russia
3
Institute for Regenerative Medicine, Lomonosov Moscow State University, 119191 Moscow, Russia
4
Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan
5
Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu 300, Taiwan
*
Author to whom correspondence should be addressed.
This paper represents part of Ph.D. thesis.
Int. J. Mol. Sci. 2019, 20(12), 3088; https://doi.org/10.3390/ijms20123088
Received: 30 April 2019 / Revised: 19 June 2019 / Accepted: 20 June 2019 / Published: 24 June 2019
(This article belongs to the Special Issue Hepatocyte Growth Factor (HGF), II)
Cell therapy remains a promising approach for the treatment of cardiovascular diseases. In this regard, the contemporary trend is the development of methods to overcome low cell viability and enhance their regenerative potential. In the present study, we evaluated the therapeutic potential of gene-modified adipose-derived stromal cells (ADSC) that overexpress hepatocyte growth factor (HGF) in a mice hind limb ischemia model. Angiogenic and neuroprotective effects were assessed following ADSC transplantation in suspension or in the form of cell sheet. We found superior blood flow restoration, tissue vascularization and innervation, and fibrosis reduction after transplantation of HGF-producing ADSC sheet compared to other groups. We suggest that the observed effects are determined by pleiotropic effects of HGF, along with the multifactorial paracrine action of ADSC which remain viable and functionally active within the engineered cell construct. Thus, we demonstrated the high therapeutic potential of the utilized approach for skeletal muscle recovery after ischemic damage associated with complex tissue degenerative effects. View Full-Text
Keywords: hepatocyte growth factor; adipose derived stromal cells; cell sheet; hind limb ischemia; skeletal muscle regeneration; angiogenesis; reinnervation hepatocyte growth factor; adipose derived stromal cells; cell sheet; hind limb ischemia; skeletal muscle regeneration; angiogenesis; reinnervation
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Boldyreva, M.A.; Shevchenko, E.K.; Molokotina, Y.D.; Makarevich, P.I.; Beloglazova, I.B.; Zubkova, E.S.; Dergilev, K.V.; Tsokolaeva, Z.I.; Penkov, D.; Hsu, M.-N.; Hu, Y.-C.; Parfyonova, Y.V. Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle. Int. J. Mol. Sci. 2019, 20, 3088.

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