Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition

Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Duesseldorf, Leibniz Center for Diabetes Research; 40225 Duesseldorf, Germany

German Center for Diabetes Research (DZD), Partner Duesseldorf, 40225 Duesseldorf, Germany

Institute for Molecular Medicine, University Hospital Duesseldorf, Heinrich Heine University Duesseldorf, 40225 Duesseldorf, Germany

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Heinrich-Heine-University Duesseldorf, Molecular Proteomics Laboratory, BMFZ, 40225 Duesseldorf, Germany

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Clinical Research Centre, Department of Internal Medicine I, University Hospital Aachen, 52074 Aachen, Germany

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2019, 20(10), 2559; https://doi.org/10.3390/ijms20102559

Received: 30 April 2019 / Revised: 21 May 2019 / Accepted: 22 May 2019 / Published: 24 May 2019

(This article belongs to the Special Issue Adipokines 2.0)

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Abstract

Adipocyte and hepatic lipid metabolism govern whole-body metabolic homeostasis, whereas a disbalance of de novo lipogenesis (DNL) in fat and liver might lead to obesity, with severe co-morbidities. Nevertheless, some obese people are metabolically healthy, but the “protective” mechanisms are not yet known in detail. Especially, the adipocyte-derived molecular mediators that indicate adipose functionality are poorly understood. We studied transgenic mice (alb-SREBP-1c) with a “healthy” obese phenotype, and obob mice with hyperphagia-induced “sick” obesity to analyze the impact of the tissue-specific DNL on the secreted proteins, i.e., the adipokinome, of the primary adipose cells by label-free proteomics. Compared to the control mice, adipose DNL is reduced in both obese mouse models. In contrast, the hepatic DNL is reduced in obob but elevated in alb-SREBP-1c mice. To investigate the relationship between lipid metabolism and adipokinomes, we formulated the “liver-to-adipose-tissue DNL” ratio. Knowledge-based analyses of these results revealed adipocyte functionality with proteins, which was involved in tissue remodeling or metabolism in the alb-SREBP-1c mice and in the control mice, but mainly in fibrosis in the obob mice. The adipokinome in “healthy” obesity is similar to that in a normal condition, but it differs from that in “sick” obesity, whereas the serum lipid patterns reflect the “liver-to-adipose-tissue DNL” ratio and are associated with the adipokinome signature. View Full-Text

Keywords: Nonalcoholic fatty liver disease; fatty liver; free fatty acids; label-free proteomic profiling; adipokine; obesity; visceral fat; sick fat

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Knebel, B.; Fahlbusch, P.; Poschmann, G.; Dille, M.; Wahlers, N.; Stühler, K.; Hartwig, S.; Lehr, S.; Schiller, M.; Jacob, S.; Kettel, U.; Müller-Wieland, D.; Kotzka, J. Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition. Int. J. Mol. Sci. 2019, 20, 2559. https://doi.org/10.3390/ijms20102559

AMA Style

Knebel B, Fahlbusch P, Poschmann G, Dille M, Wahlers N, Stühler K, Hartwig S, Lehr S, Schiller M, Jacob S, Kettel U, Müller-Wieland D, Kotzka J. Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition. International Journal of Molecular Sciences. 2019; 20(10):2559. https://doi.org/10.3390/ijms20102559

Chicago/Turabian Style

Knebel, Birgit, Pia Fahlbusch, Gereon Poschmann, Matthias Dille, Natalie Wahlers, Kai Stühler, Sonja Hartwig, Stefan Lehr, Martina Schiller, Sylvia Jacob, Ulrike Kettel, Dirk Müller-Wieland, and Jörg Kotzka. 2019. "Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition" International Journal of Molecular Sciences 20, no. 10: 2559. https://doi.org/10.3390/ijms20102559

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Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition

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