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Open AccessArticle

Cashew Tree Pollen: An Unknown Source of IgE-Reactive Molecules

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Disciplina de Imunologia Clínica e Alergia, Faculdade de Medicina da Universidade de Sao Paulo, São Paulo 01246-903, Brazil
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UNIFOR, Universidade de Fortaleza, Fortaleza 60811-905, Brazil
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Department of Biosciences, Paris-Lodron-University of Salzburg, 5020 Salzburg, Austria
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Serviço de Imunologia Clinica e Alergia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil
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ENT Research Lab, Department of Otorhinolaryngology—Head and Neck Surgery, Federal University of Sao Paulo, Sao Paulo 04021-001, Brazil
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Department of Biological Chemistry, John Innes Centre, Norwich NR4 7UH, UK
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Disciplina de Imunologia Clinica e Alergia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil
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Laboratorio de Imunologia, Instituto do Coracao, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil
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Institute for Investigation in Immunology (iii), INCT, Sao Paulo 01246-903, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(10), 2397; https://doi.org/10.3390/ijms20102397
Received: 21 March 2019 / Revised: 20 April 2019 / Accepted: 2 May 2019 / Published: 15 May 2019
(This article belongs to the Section Molecular Immunology)
Pollinosis is sub-diagnosed and rarely studied in tropical countries. Cashew tree pollen has been reported as an allergen source although the knowledge of its immunoglobulin E (IgE)-reactive molecules is lacking. Therefore, this work aimed to identify IgE-reactive molecules and provide a proteomic profile of this pollen. From the 830 proteins identified by shotgun analysis, 163 were annotated to gene ontology, and a list of 39 proteins filtered for high confidence was submitted to the Allfam database where nine were assigned to allergenic families. Thus, 12 patients from the northeast of Brazil with persistent allergic rhinitis and aggravation of symptoms during cashew flowering season were selected. Using a 2D-based approach, we identified 20 IgE-reactive proteins, four already recognized as allergens, including a homolog of the birch isoflavone-reductase (Bet v 6). IgE-reactivity against the extract in native form was confirmed for five patients in ELISA, with three being positive for Bet v 6. Herein, we present a group of patients with rhinitis exposed to cashew tree pollen with the first description of IgE-binding proteins and a proteomic profile of the whole pollen. Cashew tree pollen is considered an important trigger of rhinitis symptoms in clinical practice in the northeast of Brazil, and the elucidation of its allergenic molecules can improve the diagnostics and treatment for allergic patients. View Full-Text
Keywords: novel allergens; Brazil; pollinosis; proteome; shotgun analysis; aeroallergens novel allergens; Brazil; pollinosis; proteome; shotgun analysis; aeroallergens
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Danella Figo, D.; De Amicis, K.; Neiva Santos de Aquino, D.; Pomiecinski, F.; Gadermaier, G.; Briza, P.; Eduardo Santos Galvão, C.; Bussador do Amaral, J.; de Oliveira Martins, C.; Fernandes Morato Castro, F.; Kalil, J.; Souza Santos, K. Cashew Tree Pollen: An Unknown Source of IgE-Reactive Molecules. Int. J. Mol. Sci. 2019, 20, 2397.

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