Protective Effect of Colla corii asini against Lung Injuries Induced by Intratracheal Instillation of Artificial Fine Particles in Rats
Abstract
:1. Introduction
2. Results
2.1. Colla corii asini Protects Rats from aPM2.5-Induced Lung Function Changes
2.2. Effect of Colla corii asini on aPM2.5-Induced Alteration of T Lymphocyte Subsets
2.3. Effect of Colla corii asini on Cell Counts and Percentages
2.4. Effect of Colla corii asini on Protein and mRNA Expression of TNF-α, IL-1β and IL-10
2.5. Colla corii asini Inhibits Pathologic Changes and Lung Injury in Rats
2.6. Role of Colla corii asini in Protection against aPM2.5-Induced Alteration of Amino Metabolic Profile
3. Discussion
4. Materials and Methods
4.1. Chemical Reagents
4.2. Animal Handling
4.3. Rat model of Intratracheal Instillation of aPM2.5 and the Administration of Colla corii asini
4.4. Pulmonary Function Measurement
4.5. T Lymphocyte Phenotype Analysis of Whole Blood
4.6. Bronchoalveolar Lavage Fluid Collection and Analysis
4.7. Lung Histopathology Analysis
4.8. LC-MS/MS-Based Metabolomics Analysis
4.9. Quantitative Reverse-Transcriptase DNA Polymerase Chain Reaction (qRT-PCR) Analysis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
aPM | Artificial particular matter |
ACTB | β-actin |
BALF | Bronchoalveolar lavage fluid |
LC-MS/MS | Liquid chromatograph-mass spectrometer/ mass spectrometer |
IL | Interleukin |
References
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Group | Colla corii asini (g/kg) | Number | CD3+ (%) | CD4+ (%) | CD8+ (%) | CD4+/CD8+ |
---|---|---|---|---|---|---|
Control | - | 8 | 55.29 ± 9.46 | 44.63 ± 8.89 | 10.26 ± 3.31 | 4.84 ± 2.06 |
aPM2.5 | - | 8 | 54.69 ± 5.52 | 44.44 ± 5.50 | 10.16 ± 4.56 | 5.54 ± 3.09 |
Low-dose group | 1 | 8 | 51.28 ± 5.26 | 44.36 ± 5.49 | 6.18 ± 2.55 #,* | 8.48 ± 3.70 # |
Mid-dose group | 2 | 8 | 58.44 ± 8.44 | 48.14 ± 10.1 | 7.01 ± 4.83 | 8.22 ± 3.47 # |
High-dose group | 5 | 8 | 54.18 ± 4.79 | 47.07 ± 5.08 | 6.79 ± 2.93 | 8.95 ± 5.98 # |
Gene Name | Forward Primer | Reverse Primer |
---|---|---|
TNF-α | ACCAGCAGATGGGCTGTA | CTCCTGGTATGAAATGGCAA |
IL-1β | CAAGGAGAGACAAGCAACG | TTCATCACACAGGACAGGTAT |
IL-10 | ACTGGACTGCAGGACATA | TTTGGAGAGAGGTACAAACGA |
iNOS | TTGCTTCTGTGCTAATGCG | TCCGACTTTCCTGTCTCAGTA |
Arg-1 | TCGTACTGTGAACACGGC | GGTAGTCAGTCTCTGGCTTAT |
ACTB | CCACCATGTACCCAGGCATT | CGGACTCATCGTACTCCTGC |
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Liu, T.; Zhang, P.; Ling, Y.; Hu, G.; Gu, J.; Yang, H.; Wei, J.; Wang, A.; Jin, H. Protective Effect of Colla corii asini against Lung Injuries Induced by Intratracheal Instillation of Artificial Fine Particles in Rats. Int. J. Mol. Sci. 2019, 20, 55. https://doi.org/10.3390/ijms20010055
Liu T, Zhang P, Ling Y, Hu G, Gu J, Yang H, Wei J, Wang A, Jin H. Protective Effect of Colla corii asini against Lung Injuries Induced by Intratracheal Instillation of Artificial Fine Particles in Rats. International Journal of Molecular Sciences. 2019; 20(1):55. https://doi.org/10.3390/ijms20010055
Chicago/Turabian StyleLiu, Tiantian, Piaopiao Zhang, Yahao Ling, Guang Hu, Jianjun Gu, Hong Yang, Jinfeng Wei, Aiping Wang, and Hongtao Jin. 2019. "Protective Effect of Colla corii asini against Lung Injuries Induced by Intratracheal Instillation of Artificial Fine Particles in Rats" International Journal of Molecular Sciences 20, no. 1: 55. https://doi.org/10.3390/ijms20010055