Next Article in Journal
Comparative Study of the Molecular Basis of Pathogenicity of M. bovis Strains in a Mouse Model
Previous Article in Journal
Diet-Independent Correlations between Bacteria and Dysfunction of Gut, Adipose Tissue, and Liver: A Comprehensive Microbiota Analysis in Feces and Mucosa of the Ileum and Colon in Obese Mice with NAFLD
Previous Article in Special Issue
Dissection of the Structural Features of a Fungicidal Antibody-Derived Peptide
Article Menu
Issue 1 (January-1) cover image

Export Article

Open AccessArticle

Identification and In Vivo Characterisation of Cardioactive Peptides in Drosophila melanogaster

Department of Zoology and Developmental Biology, University of Osnabrück, Barbarastraße 11, 49076 Osnabrück, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(1), 2; https://doi.org/10.3390/ijms20010002
Received: 2 November 2018 / Accepted: 22 November 2018 / Published: 20 December 2018
(This article belongs to the Special Issue Peptides for Health Benefits)
  |  
PDF [1789 KB, uploaded 20 December 2018]
  |  

Abstract

Neuropeptides and peptide hormones serve as critical regulators of numerous biological processes, including development, growth, reproduction, physiology, and behaviour. In mammals, peptidergic regulatory systems are complex and often involve multiple peptides that act at different levels and relay to different receptors. To improve the mechanistic understanding of such complex systems, invertebrate models in which evolutionarily conserved peptides and receptors regulate similar biological processes but in a less complex manner have emerged as highly valuable. Drosophila melanogaster represents a favoured model for the characterisation of novel peptidergic signalling events and for evaluating the relevance of those events in vivo. In the present study, we analysed a set of neuropeptides and peptide hormones for their ability to modulate cardiac function in semi-intact larval Drosophila melanogaster. We identified numerous peptides that significantly affected heart parameters such as heart rate, systolic and diastolic interval, rhythmicity, and contractility. Thus, peptidergic regulation of the Drosophila heart is not restricted to chronotropic adaptation but also includes inotropic modulation. By specifically interfering with the expression of corresponding peptides in transgenic animals, we assessed the in vivo relevance of the respective peptidergic regulation. Based on the functional conservation of certain peptides throughout the animal kingdom, the identified cardiomodulatory activities may be relevant not only to proper heart function in Drosophila, but also to corresponding processes in vertebrates, including humans. View Full-Text
Keywords: endocrine signalling; dorsal vessel; heart function; heart physiology; neuropeptides; peptide hormones; peptide signalling endocrine signalling; dorsal vessel; heart function; heart physiology; neuropeptides; peptide hormones; peptide signalling
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Schiemann, R.; Lammers, K.; Janz, M.; Lohmann, J.; Paululat, A.; Meyer, H. Identification and In Vivo Characterisation of Cardioactive Peptides in Drosophila melanogaster. Int. J. Mol. Sci. 2019, 20, 2.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top