Next Article in Journal
The Ribosome as a Missing Link in Prebiotic Evolution III: Over-Representation of tRNA- and rRNA-Like Sequences and Plieofunctionality of Ribosome-Related Molecules Argues for the Evolution of Primitive Genomes from Ribosomal RNA Modules
Next Article in Special Issue
In Situ Monitoring of Bacteria under Antimicrobial Stress Using 31P Solid-State NMR
Previous Article in Journal
Druggable Targets in Cyclic Nucleotide Signaling Pathways in Apicomplexan Parasites and Kinetoplastids against Disabling Protozoan Diseases in Humans
Previous Article in Special Issue
In-Cell NMR Study of Tau and MARK2 Phosphorylated Tau
Article Menu
Issue 1 (January-1) cover image

Export Article

Open AccessReview
Int. J. Mol. Sci. 2019, 20(1), 139; https://doi.org/10.3390/ijms20010139

Applications of In-Cell NMR in Structural Biology and Drug Discovery

Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis way, Nanos, #03-01, Singapore 138669, Singapore
Received: 26 November 2018 / Revised: 24 December 2018 / Accepted: 29 December 2018 / Published: 2 January 2019
(This article belongs to the Special Issue In-Cell NMR Spectroscopy: Biomolecular Structure and Function)
Full-Text   |   PDF [1518 KB, uploaded 2 January 2019]   |  

Abstract

In-cell nuclear magnetic resonance (NMR) is a method to provide the structural information of a target at an atomic level under physiological conditions and a full view of the conformational changes of a protein caused by ligand binding, post-translational modifications or protein–protein interactions in living cells. Previous in-cell NMR studies have focused on proteins that were overexpressed in bacterial cells and isotopically labeled proteins injected into oocytes of Xenopus laevis or delivered into human cells. Applications of in-cell NMR in probing protein modifications, conformational changes and ligand bindings have been carried out in mammalian cells by monitoring isotopically labeled proteins overexpressed in living cells. The available protocols and successful examples encourage wide applications of this technique in different fields such as drug discovery. Despite the challenges in this method, progress has been made in recent years. In this review, applications of in-cell NMR are summarized. The successful applications of this method in mammalian and bacterial cells make it feasible to play important roles in drug discovery, especially in the step of target engagement. View Full-Text
Keywords: in-cell NMR; protein structure; protein dynamics; drug discovery; target engagement; protein modification in-cell NMR; protein structure; protein dynamics; drug discovery; target engagement; protein modification
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Kang, C. Applications of In-Cell NMR in Structural Biology and Drug Discovery. Int. J. Mol. Sci. 2019, 20, 139.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top