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Int. J. Mol. Sci. 2018, 19(9), 2764; https://doi.org/10.3390/ijms19092764

Comparison of Antibacterial Adhesion When Salivary Pellicle Is Coated on Both Poly(2-hydroxyethyl-methacrylate)- and Polyethylene-glycol-methacrylate-grafted Poly(methyl methacrylate)

1
Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University and National Taiwan University Hospital, No.1, Changde St., Jhongjheng District, Taipei 100, Taiwan
2
Department of Chemical Engineering, Chung Yuan Christian University, 200, Chung Pei Rd., Chung Li 320, Taiwan
3
Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan
*
Author to whom correspondence should be addressed.
Received: 28 August 2018 / Revised: 9 September 2018 / Accepted: 11 September 2018 / Published: 14 September 2018
(This article belongs to the Section Materials Science)
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Abstract

Although poly(2-hydroxyethyl methacrylate) (pHEMA) and polyethylene glycol methacrylate (PEGMA) have been demonstrated to inhibit bacterial adhesion, no study has compared antibacterial adhesion when salivary pellicle is coated on polymethyl methacrylate (PMMA) grafted with pHEMA and on PMMA grafted with PEGMA. In this study, PMMA discs were fabricated from a commercial orthodontic acrylic resin system (Ortho-Jet). Attenuated total reflection-Fourier transform infrared spectra taken before and after grafting confirmed that pHEMA and PEGMA were successfully grafted on PMMA. Contact angle measurements revealed PMMA-pHEMA to be the most hydrophilic, followed by PMMA-PEGMA, and then by PMMA. Zeta potential analysis revealed the most negative surface charges on PMMA-PEGMA, followed by PMMA-pHEMA, and then by PMMA. Confocal laser scanning microscopy showed green fluorescence in the background, indicating images that influenced the accuracy of the quantification of live bacteria. Both the optical density value measured at 600 nm and single plate-serial dilution spotting showed that pHEMA was more effective than PEGMA against Escherichia coli and Streptococcus mutans, although the difference was not significant. Therefore, the grafting of pHEMA and PEGMA separately on PMMA is effective against bacterial adhesion, even after the grafted PMMA were coated with salivary pellicle. Surface hydrophilicity, bactericidality, and Coulomb repulsion between the negatively charged bacteria and the grafted surface contributed to the effectiveness. View Full-Text
Keywords: anti-adhesion; antibacterial adhesion; protein adsorption; pellicle coating; dentistry; orthodontic; biocompatibility; serial dilution spotting; prostheses anti-adhesion; antibacterial adhesion; protein adsorption; pellicle coating; dentistry; orthodontic; biocompatibility; serial dilution spotting; prostheses
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Lee, B.-S.; Chen, Y.-J.; Wei, T.-C.; Ma, T.-L.; Chang, C.-C. Comparison of Antibacterial Adhesion When Salivary Pellicle Is Coated on Both Poly(2-hydroxyethyl-methacrylate)- and Polyethylene-glycol-methacrylate-grafted Poly(methyl methacrylate). Int. J. Mol. Sci. 2018, 19, 2764.

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