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Discovery of lahS as a Global Regulator of Environmental Adaptation and Virulence in Aeromonas hydrophila

Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(9), 2709;
Received: 18 August 2018 / Revised: 3 September 2018 / Accepted: 4 September 2018 / Published: 11 September 2018
(This article belongs to the Special Issue Microbial Virulence Factors)
PDF [4118 KB, uploaded 11 September 2018]


Aeromonas hydrophila is an important aquatic microorganism that can cause fish hemorrhagic septicemia. In this study, we identified a novel LysR family transcriptional regulator (LahS) in the A. hydrophila Chinese epidemic strain NJ-35 from a library of 947 mutant strains. The deletion of lahS caused bacteria to exhibit significantly decreased hemolytic activity, motility, biofilm formation, protease production, and anti-bacterial competition ability when compared to the wild-type strain. In addition, the determination of the fifty percent lethal dose (LD50) in zebrafish demonstrated that the lahS deletion mutant (ΔlahS) was highly attenuated in virulence, with an approximately 200-fold increase in LD50 observed as compared with that of the wild-type strain. However, the ΔlahS strain exhibited significantly increased antioxidant activity (six-fold). Label-free quantitative proteome analysis resulted in the identification of 34 differentially expressed proteins in the ΔlahS strain. The differentially expressed proteins were involved in flagellum assembly, metabolism, redox reactions, and cell density induction. The data indicated that LahS might act as a global regulator to directly or indirectly regulate various biological processes in A. hydrophila NJ-35, contributing to a greater understanding the pathogenic mechanisms of A. hydrophila. View Full-Text
Keywords: Aeromonas hydrophila; LysR-family; ΔlahS; global regulator; virulence Aeromonas hydrophila; LysR-family; ΔlahS; global regulator; virulence

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Dong, Y.; Wang, Y.; Liu, J.; Ma, S.; Awan, F.; Lu, C.; Liu, Y. Discovery of lahS as a Global Regulator of Environmental Adaptation and Virulence in Aeromonas hydrophila. Int. J. Mol. Sci. 2018, 19, 2709.

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