Int. J. Mol. Sci. 2018, 19(7), 1969; https://doi.org/10.3390/ijms19071969
Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains
1
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Korea
2
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
*
Author to whom correspondence should be addressed.
Received: 18 May 2018 / Revised: 22 June 2018 / Accepted: 5 July 2018 / Published: 6 July 2018
(This article belongs to the Section Biochemistry)
Abstract
Chlorella has great potential as a bio-factory for production of value-added compounds. To produce the desired chemicals more efficiently in Chlorella, genetic tools for modification of Chlorella need to be developed, especially an endogenous promoter. In this study, the promoter of photosystem I protein D (psaD) from Chlorella vulgaris UTEX395 was identified. Computational analysis revealed the presence of several putative cis-acting elements, including a potential core element, and light-responsive or stress-responsive elements. Gene expression analysis in heterologous expression system in Chlamydomonasreinhardtii and Nicotianabenthamiana showed that CvpsaD promoter can be used to drive the expression of genes. Functional analysis of this promoter suggested that the initiator element (Inr) is important for its function (i.e., TATA-less promoter) and that an additional factor (e.g., downstream of the transcriptional start site) might be needed for light response. We have shown that the CvpsaD promoter is functional, but not sufficiently strong, both in microalgae and higher plant. View Full-TextKeywords:
Chlorella vulgaris; native promoter; photosystem I protein D (psaD); heterologous expression
▼
Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Share & Cite This Article
MDPI and ACS Style
Kim, J.; Liu, L.; Hu, Z.; Jin, E. Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains. Int. J. Mol. Sci. 2018, 19, 1969.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
Related Articles
Article Metrics
Comments
[Return to top]
Int. J. Mol. Sci.
EISSN 1422-0067
Published by MDPI AG, Basel, Switzerland
RSS
E-Mail Table of Contents Alert