Next Article in Journal
Unravelling HDL—Looking beyond the Cholesterol Surface to the Quality Within
Previous Article in Journal
Schisandra chinensis Fructus and Its Active Ingredients as Promising Resources for the Treatment of Neurological Diseases
Open AccessArticle

Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains

1
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Korea
2
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(7), 1969; https://doi.org/10.3390/ijms19071969
Received: 18 May 2018 / Revised: 22 June 2018 / Accepted: 5 July 2018 / Published: 6 July 2018
(This article belongs to the Section Biochemistry)
Chlorella has great potential as a bio-factory for production of value-added compounds. To produce the desired chemicals more efficiently in Chlorella, genetic tools for modification of Chlorella need to be developed, especially an endogenous promoter. In this study, the promoter of photosystem I protein D (psaD) from Chlorella vulgaris UTEX395 was identified. Computational analysis revealed the presence of several putative cis-acting elements, including a potential core element, and light-responsive or stress-responsive elements. Gene expression analysis in heterologous expression system in Chlamydomonasreinhardtii and Nicotianabenthamiana showed that CvpsaD promoter can be used to drive the expression of genes. Functional analysis of this promoter suggested that the initiator element (Inr) is important for its function (i.e., TATA-less promoter) and that an additional factor (e.g., downstream of the transcriptional start site) might be needed for light response. We have shown that the CvpsaD promoter is functional, but not sufficiently strong, both in microalgae and higher plant. View Full-Text
Keywords: Chlorella vulgaris; native promoter; photosystem I protein D (psaD); heterologous expression Chlorella vulgaris; native promoter; photosystem I protein D (psaD); heterologous expression
Show Figures

Figure 1

MDPI and ACS Style

Kim, J.; Liu, L.; Hu, Z.; Jin, E. Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains. Int. J. Mol. Sci. 2018, 19, 1969. https://doi.org/10.3390/ijms19071969

AMA Style

Kim J, Liu L, Hu Z, Jin E. Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains. International Journal of Molecular Sciences. 2018; 19(7):1969. https://doi.org/10.3390/ijms19071969

Chicago/Turabian Style

Kim, Jongrae; Liu, Linpo; Hu, Zanmin; Jin, EonSeon. 2018. "Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains" Int. J. Mol. Sci. 19, no. 7: 1969. https://doi.org/10.3390/ijms19071969

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop