STIM1 Knockout Enhances PDGF-Mediated Ca2+ Signaling through Upregulation of the PDGFR–PLCγ–STIM2 Cascade
Abstract
:1. Introduction
2. Results
2.1. Stromal Interaction Molecule 1 (STIM1) Knockout Represses Store-Operated Ca2+ Entry (SOCE) in Mouse Embryonic Fibroblasts (MEF) Cells
2.2. PDGF-BB Induced Significant Ca2+ Elevation in STIM1 Knockout MEF Cells
2.3. Upregulation and Activation of PDGFRα, PDGFRβ, and Phospholipase C Gamma (PLCγ) in MEF-STIM1−/− Cells
2.4. PDGF-BB-Mediated ER Store-Depletion Activates STIM2 Translocation and Puncta Formation
2.5. SOCE Inhibitors Decrease PDGF-BB-Induced Ca2+ Elevation in MEF-STIM1−/− Cells
2.6. PDGFR–PLCγ–STIM2 Signaling Induces Cell Migration in MEF-STIM1−/− Cells.
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Reagents
4.2. Single-Cell Ca2+ Measurement
4.3. Immunoblotting Analyses
4.4. Immunofluorescence Staining and Confocal Microscopy
4.5. Wound Healing Assay
4.6. Cell Proliferation Analysis
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
MEF | Mouse embryonic fibroblasts |
PDGF | Platelet-derived growth factor |
PDGFR | Platelet-derived growth factor receptor |
SOCE | Store-operated Ca2+ entry |
STIM1 | Stromal interaction molecule 1 |
Orai1 | Ca2+ release-activated calcium modulator 1 |
TRPC1 | Transient receptor potential canonical 1 |
PLCγ | Phospholipase C gamma |
RTK | Receptor tyrosine kinase |
CREB | cAMP response element binding protein |
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Huang, T.-Y.; Lin, Y.-H.; Chang, H.-A.; Yeh, T.-Y.; Chang, Y.-H.; Chen, Y.-F.; Chen, Y.-C.; Li, C.-C.; Chiu, W.-T. STIM1 Knockout Enhances PDGF-Mediated Ca2+ Signaling through Upregulation of the PDGFR–PLCγ–STIM2 Cascade. Int. J. Mol. Sci. 2018, 19, 1799. https://doi.org/10.3390/ijms19061799
Huang T-Y, Lin Y-H, Chang H-A, Yeh T-Y, Chang Y-H, Chen Y-F, Chen Y-C, Li C-C, Chiu W-T. STIM1 Knockout Enhances PDGF-Mediated Ca2+ Signaling through Upregulation of the PDGFR–PLCγ–STIM2 Cascade. International Journal of Molecular Sciences. 2018; 19(6):1799. https://doi.org/10.3390/ijms19061799
Chicago/Turabian StyleHuang, Tzu-Yu, Yi-Hsin Lin, Heng-Ai Chang, Tzu-Ying Yeh, Ya-Han Chang, Yi-Fan Chen, Ying-Chi Chen, Chun-Chun Li, and Wen-Tai Chiu. 2018. "STIM1 Knockout Enhances PDGF-Mediated Ca2+ Signaling through Upregulation of the PDGFR–PLCγ–STIM2 Cascade" International Journal of Molecular Sciences 19, no. 6: 1799. https://doi.org/10.3390/ijms19061799