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Int. J. Mol. Sci. 2018, 19(6), 1733; https://doi.org/10.3390/ijms19061733

Overexpression of BraLTP2, a Lipid Transfer Protein of Brassica napus, Results in Increased Trichome Density and Altered Concentration of Secondary Metabolites

Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Received: 10 May 2018 / Revised: 28 May 2018 / Accepted: 28 May 2018 / Published: 12 June 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Plant non-specific lipid transfer proteins (nsLTPs) belong to a large multigene family that possesses complex physiological functions. Trichomes are present on the aerial surfaces of most plants and include both glandular secretory hairs and non-glandular hairs. In this study, BraLTP2 was isolated from Brassica rapa (B. rapa) and its function was characterized in the important oilseed crop Brassica napus (B. napus). B. rapa lipid transfer protein 2 (BraLTP2) belongs to the little-known Y class of nsLTPs and encodes a predicted secretory protein. In ProBraLTP2::GUS (β-glucuronidase) transgenic plants, strong GUS activity was observed in young leaves and roots, while low activity was observed in the anther. It is noteworthy that strong GUS activity was observed in trichomes of the first four leaves of 4-week-old and 8-week-old seedings, however, it disappeared in 12-week-old seedings. In transgenic plants expressing a BraLTP2::GFP (green fluorescent protein) fusion protein, GFP fluorescence localized in the extracellular space of epidermal cells and trichomes. Overexpression of BraLTP2 in B. napus caused an increase in trichome number and altered the accumulation of secondary metabolites in leaves, including 43 upregulated secondary metabolites. Moreover, transgenic plants showed significantly increased activities of antioxidant enzymes. These results suggest that BraLTP2, a new nsLTP gene, may play a role in trichome development and the accumulation of secondary metabolites. View Full-Text
Keywords: BraLTP2; overexpression; trichome development; secondary metabolites; antioxidant enzymes; Brassica napus BraLTP2; overexpression; trichome development; secondary metabolites; antioxidant enzymes; Brassica napus
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Tian, N.; Liu, F.; Wang, P.; Yan, X.; Gao, H.; Zeng, X.; Wu, G. Overexpression of BraLTP2, a Lipid Transfer Protein of Brassica napus, Results in Increased Trichome Density and Altered Concentration of Secondary Metabolites. Int. J. Mol. Sci. 2018, 19, 1733.

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