PIEZO1 Channel Is a Potential Regulator of Synovial Sarcoma Cell-Viability
AbstractDetection of mechanical stress is essential for diverse biological functions including touch, audition, and maintenance of vascular myogenic tone. PIEZO1, a mechano-sensing cation channel, is widely expressed in neuronal and non-neuronal cells and is expected to be involved in important biological functions. Here, we examined the possibility that PIEZO1 is involved in the regulation of synovial sarcoma cell-viability. Application of a PIEZO1 agonist Yoda1 effectively induced Ca2+ response and cation channel currents in PIEZO1-expressing HEK (HEK-Piezo1) cells and synovial sarcoma SW982 (SW982) cells. Mechanical stress, as well as Yoda1, induced the activity of an identical channel of conductance with 21.6 pS in HEK-Piezo1 cells. In contrast, Yoda1 up to 10 μM had no effects on membrane currents in HEK cells without transfecting PIEZO1. A knockdown of PIEZO1 with siRNA in SW982 cells abolished Yoda1-induced Ca2+ response and significantly reduced cell cell-viability. Because PIEZO1 is highly expressed in SW982 cells and its knockdown affects cell-viability, this gene is a potential target against synovial sarcoma. View Full-Text
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Suzuki, T.; Muraki, Y.; Hatano, N.; Suzuki, H.; Muraki, K. PIEZO1 Channel Is a Potential Regulator of Synovial Sarcoma Cell-Viability. Int. J. Mol. Sci. 2018, 19, 1452.
Suzuki T, Muraki Y, Hatano N, Suzuki H, Muraki K. PIEZO1 Channel Is a Potential Regulator of Synovial Sarcoma Cell-Viability. International Journal of Molecular Sciences. 2018; 19(5):1452.Chicago/Turabian Style
Suzuki, Takahisa; Muraki, Yukiko; Hatano, Noriyuki; Suzuki, Hiroka; Muraki, Katsuhiko. 2018. "PIEZO1 Channel Is a Potential Regulator of Synovial Sarcoma Cell-Viability." Int. J. Mol. Sci. 19, no. 5: 1452.
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