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Int. J. Mol. Sci. 2018, 19(4), 1209;

Comparative microRNA-seq Analysis Depicts Candidate miRNAs Involved in Skin Color Differentiation in Red Tilapia

Freshwater Fisheries Research Centre of Chinese Academy of Fishery Sciences, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Wuxi 214081, China
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China
Author to whom correspondence should be addressed.
Received: 9 March 2018 / Revised: 30 March 2018 / Accepted: 13 April 2018 / Published: 16 April 2018
(This article belongs to the Section Biochemistry)
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Differentiation and variation in body color has been a growing limitation to the commercial value of red tilapia. Limited microRNA (miRNA) information is available on skin color differentiation and variation in fish so far. In this study, a high-throughput Illumina sequencing of sRNAs was conducted on three color varieties of red tilapia and 81,394,491 raw reads were generated. A total of 158 differentially expressed miRNAs (|log2(fold change)| ≥ 1 and q-value ≤ 0.001) were identified. Target prediction and functional analysis of color-related miRNAs showed that a variety of putative target genes—including slc7a11, mc1r and asip—played potential roles in pigmentation. Moreover; the miRNA-mRNA regulatory network was illustrated to elucidate the pigmentation differentiation, in which miR-138-5p and miR-722 were predicted to play important roles in regulating the pigmentation process. These results advance our understanding of the molecular mechanisms of skin pigmentation differentiation in red tilapia. View Full-Text
Keywords: miR-seq; skin color; red tilapia; pigment; comparative analysis miR-seq; skin color; red tilapia; pigment; comparative analysis

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Wang, L.; Zhu, W.; Dong, Z.; Song, F.; Dong, J.; Fu, J. Comparative microRNA-seq Analysis Depicts Candidate miRNAs Involved in Skin Color Differentiation in Red Tilapia. Int. J. Mol. Sci. 2018, 19, 1209.

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