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Int. J. Mol. Sci. 2018, 19(4), 1175;

Triclosan Lacks (Anti-)Estrogenic Effects in Zebrafish Cells but Modulates Estrogen Response in Zebrafish Embryos

Institut National de l’environnement Industriel et des Risques (INERIS), Unité d’Ecotoxicologie in vitro et in vivo, UMR-I SEBIO 02, BP 2, 60550 Verneuil-en-Halatte, France
Université de Bordeaux, EPOC-UMR 5805, Laboratoire de Physico- et Toxico-Chimie de l’environnement (LPTC), 33405 Talence, France
Author to whom correspondence should be addressed.
Received: 9 February 2018 / Revised: 6 April 2018 / Accepted: 10 April 2018 / Published: 12 April 2018
(This article belongs to the Special Issue Molecular Pathways of Estrogen Receptor Action)
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Triclosan (TCS), an antimicrobial agent widely found in the aquatic environment, is suspected to act as an endocrine disrupting compound, however mechanistic information is lacking in regards to aquatic species. This study assessed the ability of TCS to interfere with estrogen receptor (ER) transcriptional activity, in zebrafish-specific in vitro and in vivo reporter gene assays. We report that TCS exhibits a lack of either agonistic or antagonistic effects on a panel of ER-expressing zebrafish (ZELH-zfERα and -zfERβ) and human (MELN) cell lines. At the organism level, TCS at concentrations of up to 0.3 µM had no effect on ER-regulated brain aromatase gene expression in transgenic cyp19a1b-GFP zebrafish embryos. At a concentration of 1 µM, TCS interfered with the E2 response in an ambivalent manner by potentializing a low E2 response (0.625 nM), but decreasing a high E2 response (10 nM). Altogether, our study suggests that while modulation of ER-regulated genes by TCS may occur in zebrafish, it does so irrespective of a direct binding and activation of zfERs. View Full-Text
Keywords: in vitro; in vivo; estrogen receptor; zebrafish; triclosan; brain aromatase in vitro; in vivo; estrogen receptor; zebrafish; triclosan; brain aromatase

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Serra, H.; Brion, F.; Porcher, J.-M.; Budzinski, H.; Aït-Aïssa, S. Triclosan Lacks (Anti-)Estrogenic Effects in Zebrafish Cells but Modulates Estrogen Response in Zebrafish Embryos. Int. J. Mol. Sci. 2018, 19, 1175.

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