Radiation-Induced Gene Expression Changes in High and Low Grade Breast Cancer Cell Types
Abstract
:1. Introduction
2. Results
2.1. Gene Expression and Pathway Analysis
2.1.1. 9 Gy-Treated Immortalized Breast Cell Lines
2.1.2. 23 Gy-Treated Immortalized Breast Cell Lines
2.1.3. 9 Gy-Treated Primary Breast Cell Cultures
2.1.4. 23 Gy-Treated Primary Breast Cell Cultures
2.2. Comparison between 9Gy and 23Gy
2.3. Comparison between 9-Gy IR Treated Immortalized and Primary Cell Lines
PCA Analysis
2.4. Network Analysis of 9 Gy DEGs in Immortalized and Primary Cells
3. Discussion
4. Materials and Methods
4.1. Cell Cultures and Irradiation
4.2. Gene Expression Analysis
4.3. Pathway Analysis
4.4. Network Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
RT | Radiation therapy |
BC | Breast Cancer |
HR | Hormone receptor |
HER2 | Human epidermal growth factor receptor |
IR | Ionizing radiation |
ER | Estrogen receptor |
PR | Progesteron receptor |
IOERT | Intraoperative electron radiotherapy |
DEG | Differentially expressed gene |
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9 Gy | 23 Gy | 9 ∩ 23 Gy | |||
---|---|---|---|---|---|
N° DEGs | N° PATHs | N° DEGs | N° PATHs | N° PATHs | |
TOTAL MCF10A | 560 | 10 | 582 | 5 | 2 |
TOTAL MCF7 | 777 | 19 | 505 | 37 | 7 |
TOTAL MDA-MB-231 | 416 | 14 | 627 | 14 | 14 |
MCF10A ∩ MCF7 | 53 | 0 | 36 | 3 | |
MCF10A ∩ MDA-MB-231 | 20 | 0 | 25 | 0 | |
MCF7 ∩ MDA-MB-231 | 32 | 0 | 47 | 12 | |
MCF10A ∩ MCF7 ∩ MDA-MB-231 | 7 | 0 | 7 | 0 | |
MCF10A-specific | 480 | 10 | 514 | 2 | |
MCF7-specific | 685 | 19 | 415 | 22 | |
MDA-MB-231-specific | 357 | 14 | 548 | 2 | |
TOTAL HMEC | 435 | 17 | 658 | 54 | 16 |
TOTAL BCpc7 | 527 | 68 | 621 | 62 | 58 |
TOTAL BCpcEMT | 687 | 43 | 753 | 35 | 33 |
HMEC ∩ BCpc7 | 23 | 1 | 68 | 21 | |
HMEC ∩ BCpcEMT | 73 | 0 | 79 | 0 | |
BCpc7 ∩ BCpcEMT | 194 | 27 | 101 | 2 | |
HMEC ∩ BCpc7 ∩ BCpcEMT | 84 | 15 | 203 | 33 | |
HMEC-specific | 255 | 1 | 308 | 0 | |
BCpc7-specific | 226 | 25 | 249 | 6 | |
BCpcEMT-specific | 336 | 1 | 370 | 0 | |
MCF10A ∩ HMEC | 11 | 1 | |||
MCF7 ∩ BCpc7 | 64 | 6 | |||
MDA-MB-231 ∩ BCpcEMT | 59 | 9 |
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Bravatà, V.; Cava, C.; Minafra, L.; Cammarata, F.P.; Russo, G.; Gilardi, M.C.; Castiglioni, I.; Forte, G.I. Radiation-Induced Gene Expression Changes in High and Low Grade Breast Cancer Cell Types. Int. J. Mol. Sci. 2018, 19, 1084. https://doi.org/10.3390/ijms19041084
Bravatà V, Cava C, Minafra L, Cammarata FP, Russo G, Gilardi MC, Castiglioni I, Forte GI. Radiation-Induced Gene Expression Changes in High and Low Grade Breast Cancer Cell Types. International Journal of Molecular Sciences. 2018; 19(4):1084. https://doi.org/10.3390/ijms19041084
Chicago/Turabian StyleBravatà, Valentina, Claudia Cava, Luigi Minafra, Francesco Paolo Cammarata, Giorgio Russo, Maria Carla Gilardi, Isabella Castiglioni, and Giusi Irma Forte. 2018. "Radiation-Induced Gene Expression Changes in High and Low Grade Breast Cancer Cell Types" International Journal of Molecular Sciences 19, no. 4: 1084. https://doi.org/10.3390/ijms19041084