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Int. J. Mol. Sci. 2018, 19(4), 1034;

The Role of Serotonin during Skin Healing in Post-Thermal Injury

Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada
Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada
Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology (NUST), H-12 Islamabad, Pakistan
Department of Surgery, University of Toronto, Toronto, ON M5T 1P5, Canada
Molecular Immunology Unit, The Institute of Infection and Immunity, St. George’s, University of London, London SW17 0RE, UK
Department of Laboratory Medicine and Pathobiology (LMP), University of Toronto, Toronto, ON M5S 1A8, Canada
Authors to whom correspondence should be addressed.
Received: 21 February 2018 / Revised: 24 March 2018 / Accepted: 26 March 2018 / Published: 29 March 2018
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Post-burn trauma significantly raises tissue serotonin concentration at the initial stages of injury, which leads us to investigate its possible role in post burn wound healing. Therefore, we planned this study to examine the role of serotonin in wound healing through in vitro and in vivo models of burn injuries. Results from in vitro analysis revealed that serotonin decreased apoptosis and increased cell survival significantly in human fibroblasts and neonatal keratinocytes. Cellular proliferation also increased significantly in both cell types. Moreover, serotonin stimulation significantly accelerated the cell migration, resulting in narrowing of the scratch zone in human neonatal keratinocytes and fibroblasts cultures. Whereas, fluoxetine (a selective serotonin reuptake inhibitor) and ketanserin (serotonin receptor 2A inhibitor) reversed these effects. Scald burn mice model (20% total body surface area) showed that endogenous serotonin improved wound healing process in control group, whereas fluoxetine and ketanserin treatments (disruptors of endogenous serotonin stimulation), resulted in poor reepithelization, bigger wound size and high alpha smooth muscle actin (α-SMA) count. All of these signs refer a prolonged differentiation state, which ultimately exhibits poor wound healing outcomes. Collectively, data showed that the endogenous serotonin pathway contributes to regulating the skin wound healing process. Hence, the results of this study signify the importance of serotonin as a potential therapeutic candidate for enhancing skin healing in burn patients. View Full-Text
Keywords: serotonin; fibroblasts; keratinocytes; migration; post thermal injury; wound healing serotonin; fibroblasts; keratinocytes; migration; post thermal injury; wound healing

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Sadiq, A.; Shah, A.; Jeschke, M.G.; Belo, C.; Qasim Hayat, M.; Murad, S.; Amini-Nik, S. The Role of Serotonin during Skin Healing in Post-Thermal Injury. Int. J. Mol. Sci. 2018, 19, 1034.

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