Next Article in Journal
Chloroplast Protein Turnover: The Influence of Extraplastidic Processes, Including Autophagy
Previous Article in Journal
Applications of Metals for Bone Regeneration
Open AccessArticle

Comparative Transcriptome Analyses Reveal Potential Mechanisms of Enhanced Drought Tolerance in Transgenic Salvia Miltiorrhiza Plants Expressing AtDREB1A from Arabidopsis

1
National Engineering Research Center of Pesticide (Tianjin), Nankai University, Tianjin 300071, China
2
College of Life Sciences, Nankai University, Tianjin 300071, China
3
School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
4
Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(3), 827; https://doi.org/10.3390/ijms19030827
Received: 27 January 2018 / Revised: 7 March 2018 / Accepted: 10 March 2018 / Published: 12 March 2018
(This article belongs to the Section Molecular Plant Sciences)
In our previous study, drought-resistant transgenic plants of Salvia miltiorrhiza were produced via overexpression of the transcription factor AtDREB1A. To unravel the molecular mechanisms underpinning elevated drought tolerance in transgenic plants, in the present study we compared the global transcriptional profiles of wild-type (WT) and AtDREB1A-expressing transgenic plants using RNA-sequencing (RNA-seq). Using cluster analysis, we identified 3904 differentially expressed genes (DEGs). Compared with WT plants, 423 unigenes were up-regulated in pRD29A::AtDREB1A-31 before drought treatment, while 936 were down-regulated and 1580 and 1313 unigenes were up- and down-regulated after six days of drought. COG analysis revealed that the ‘signal transduction mechanisms’ category was highly enriched among these DEGs both before and after drought stress. Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, DEGs associated with “ribosome”, “plant hormone signal transduction”, photosynthesis”, “plant-pathogen interaction”, “glycolysis/gluconeogenesis” and “carbon fixation” are hypothesized to perform major functions in drought resistance in AtDREB1A-expressing transgenic plants. Furthermore, the number of DEGs associated with different transcription factors increased significantly after drought stress, especially the AP2/ERF, bZIP and MYB protein families. Taken together, this study substantially expands the transcriptomic information for S. miltiorrhiza and provides valuable clues for elucidating the mechanism of AtDREB1A-mediated drought tolerance in transgenic plants. View Full-Text
Keywords: transcriptome; drought; AtDREB1A; Salvia miltiorrhiza; phytohormones; transcription factors; photosynthesis transcriptome; drought; AtDREB1A; Salvia miltiorrhiza; phytohormones; transcription factors; photosynthesis
Show Figures

Figure 1

MDPI and ACS Style

Wei, T.; Deng, K.; Wang, H.; Zhang, L.; Wang, C.; Song, W.; Zhang, Y.; Chen, C. Comparative Transcriptome Analyses Reveal Potential Mechanisms of Enhanced Drought Tolerance in Transgenic Salvia Miltiorrhiza Plants Expressing AtDREB1A from Arabidopsis. Int. J. Mol. Sci. 2018, 19, 827.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop