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Open AccessArticle

Inhibition of Intracellular ROS Accumulation by Formononetin Attenuates Cisplatin-Mediated Apoptosis in LLC-PK1 Cells

1
Department of Korean International Medicine, College of Korean Medicine, Gachon University, Seongnam 13120, Korea
2
College of Korean Medicine, Gachon University, Seongnam 13120, Korea
3
Department of Medicine, University of Ulsan College of Medicine, Seoul 05505, Korea
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(3), 813; https://doi.org/10.3390/ijms19030813
Received: 5 March 2018 / Revised: 10 March 2018 / Accepted: 10 March 2018 / Published: 12 March 2018
(This article belongs to the Section Bioactives and Nutraceuticals)
Cisplatin is a well-known anticancer drug frequently used for treating solid tumors, including ovarian, testicular, bladder, and cervical tumors. However, usage of cisplatin has been limited because of its adverse effects, particularly nephrotoxicity. Therefore, the present study sought to investigate the protective effect of formononetin against cisplatin-induced cytotoxicity in LLC-PK1 pig kidney epithelial cells as well as the anticancer effect of cisplatin in three different human cervical cancer cell lines, including HeLa, SiHa, and CaSKi cells. We first demonstrated that formononetin strongly prevented cisplatin-induced LLC-PK1 cell death. Although formononetin had no anticancer effect, it did not interrupt the anticancer effect of cisplatin in human cervical carcinoma cell lines. Furthermore, the treatment with formononetin reduced reactive oxygen species (ROS) accumulation and chromatin condensation. The percentage of Annexin V-positive cells also increased following cisplatin treatment. Finally, formononetin-inhibited c-Jun N-terminal kinase (JNK) phosphorylation, cleavage of caspase-8 and caspase-3, and the ratio of Bax to Bcl-2 increased with cisplatin. Taken together, these findings suggest that formononetin may be a possible option to prevent nephrotoxicity induced by cisplatin during treatment for cervical cancer. View Full-Text
Keywords: formononetin; nephrotoxicity; cisplatin; apoptosis; LLC-PK1 cell; cervical cancer cells formononetin; nephrotoxicity; cisplatin; apoptosis; LLC-PK1 cell; cervical cancer cells
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MDPI and ACS Style

Lee, H.; Lee, D.; Kang, K.S.; Song, J.H.; Choi, Y.-K. Inhibition of Intracellular ROS Accumulation by Formononetin Attenuates Cisplatin-Mediated Apoptosis in LLC-PK1 Cells. Int. J. Mol. Sci. 2018, 19, 813.

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