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Int. J. Mol. Sci. 2018, 19(3), 737; https://doi.org/10.3390/ijms19030737

Identification and Functional Analysis of a Novel Cytochrome P450 Gene CYP9A105 Associated with Pyrethroid Detoxification in Spodoptera exigua Hübner

1
Guangdong Engineering Research Center for Modern Eco-Agruculture and Circular Agriculture, Guangzhou 510642, China
2
Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China
3
United States Department of Agriculture-Agricultural Research Service, Natural Products Utilization Research Unit, University of Mississippi, Oxford, MS 38677, USA
4
Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China
*
Author to whom correspondence should be addressed.
Received: 12 December 2017 / Revised: 22 February 2018 / Accepted: 2 March 2018 / Published: 5 March 2018
(This article belongs to the Section Biochemistry)
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Abstract

In insects, cytochrome P450 monooxygenases (P450s or CYPs) are known to be involved in the detoxification and metabolism of insecticides, leading to increased resistance in insect populations. Spodoptera exigua is a serious polyphagous insect pest worldwide and has developed resistance to various insecticides. In this study, a novel CYP3 clan P450 gene CYP9A105 was identified and characterized from S. exigua. The cDNAs of CYP9A105 encoded 530 amino acid proteins, respectively. Quantitative real-time PCR analyses showed that CYP9A105 was expressed at all developmental stages, with maximal expression observed in fifth instar stage larvae, and in dissected fifth instar larvae the highest transcript levels were found in midguts and fat bodies. The expression of CYP9A105 in midguts was upregulated by treatments with the insecticides α-cypermethrin, deltamethrin and fenvalerate at both LC15 concentrations (0.10, 0.20 and 5.0 mg/L, respectively) and LC50 concentrations (0.25, 0.40 and 10.00 mg/L, respectively). RNA interference (RNAi) mediated silencing of CYP9A105 led to increased mortalities of insecticide-treated 4th instar S. exigua larvae. Our results suggest that CYP9A105 might play an important role in α-cypermethrin, deltamethrin and fenvalerate detoxification in S. exigua. View Full-Text
Keywords: Spodoptera exigua; cytochrome P450; pyrethroids; RNA interference; CYP9A105 Spodoptera exigua; cytochrome P450; pyrethroids; RNA interference; CYP9A105
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Wang, R.-L.; Liu, S.-W.; Baerson, S.R.; Qin, Z.; Ma, Z.-H.; Su, Y.-J.; Zhang, J.-E. Identification and Functional Analysis of a Novel Cytochrome P450 Gene CYP9A105 Associated with Pyrethroid Detoxification in Spodoptera exigua Hübner. Int. J. Mol. Sci. 2018, 19, 737.

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