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Aptamers Selected for Recognizing Amyloid β-Protein—A Case for Cautious Optimism

Division of Biomedical Science and Biochemistry, Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia
Int. J. Mol. Sci. 2018, 19(3), 668; https://doi.org/10.3390/ijms19030668
Received: 5 February 2018 / Revised: 18 February 2018 / Accepted: 22 February 2018 / Published: 27 February 2018
(This article belongs to the Special Issue Aptamers)
Aptamers are versatile oligonucleotide ligands used for molecular recognition of diverse targets. However, application of aptamers to the field of amyloid β-protein (Aβ) has been limited so far. Aβ is an intrinsically disordered protein that exists in a dynamic conformational equilibrium, presenting time-dependent ensembles of short-lived, metastable structures and assemblies that have been generally difficult to isolate and characterize. Moreover, despite understanding of potential physiological roles of Aβ, this peptide has been linked to the pathogenesis of Alzheimer disease, and its pathogenic roles remain controversial. Accumulated scientific evidence thus far highlights undesirable or nonspecific interactions between selected aptamers and different Aβ assemblies likely due to the metastable nature of Aβ or inherent affinity of RNA oligonucleotides to β-sheet-rich fibrillar structures of amyloidogenic proteins. Accordingly, lessons drawn from Aβ–aptamer studies emphasize that purity and uniformity of the protein target and rigorous characterization of aptamers’ specificity are important for realizing and garnering the full potential of aptamers selected for recognizing Aβ or other intrinsically disordered proteins. This review summarizes studies of aptamers selected for recognizing different Aβ assemblies and highlights controversies, difficulties, and limitations of such studies. View Full-Text
Keywords: Alzheimer disease; amyloid β-protein; antibodies; cross-reactions; nucleotide aptamers; oligonucleotide ligands; systematic evolution of ligands by exponential enrichment; specificity; therapeutics Alzheimer disease; amyloid β-protein; antibodies; cross-reactions; nucleotide aptamers; oligonucleotide ligands; systematic evolution of ligands by exponential enrichment; specificity; therapeutics
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Rahimi, F. Aptamers Selected for Recognizing Amyloid β-Protein—A Case for Cautious Optimism. Int. J. Mol. Sci. 2018, 19, 668.

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