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Volume 19
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10.3390/ijms19020537
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Article

Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy

by
Mélanie Desancé
1,
Romain Contentin
1,
Lélia Bertoni
2,
Tangni Gomez-Leduc
1,
Thomas Branly
1,
Sandrine Jacquet
2,
Jean-Marc Betsch
3,
Agnès Batho
1,4,
Florence Legendre
1,
Fabrice Audigié
2,
Philippe Galéra
1,* and
Magali Demoor
1,*
1
Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France
2
Center of Imaging and Research on Locomotor Affections in Equines, Ecole Vétérinaire d’Alfort, Université Paris-Est, 14430 Goustranville, France
3
Clinique Vétérinaire Equine de Méheudin, Méheudin, 61150 Ecouché, France
4
EFS Caen, 14000 Caen, France
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(2), 537; https://doi.org/10.3390/ijms19020537
Submission received: 19 January 2018 / Revised: 3 February 2018 / Accepted: 7 February 2018 / Published: 10 February 2018
(This article belongs to the Section Materials Science)
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Abstract

Cartilage engineering is a new strategy for the treatment of cartilage damage due to osteoarthritis or trauma in humans. Racehorses are exposed to the same type of cartilage damage and the anatomical, cellular, and biochemical properties of their cartilage are comparable to those of human cartilage, making the horse an excellent model for the development of cartilage engineering. Human mesenchymal stem cells (MSCs) differentiated into chondrocytes with chondrogenic factors in a biomaterial appears to be a promising therapeutic approach for direct implantation and cartilage repair. Here, we characterized equine umbilical cord blood-derived MSCs (eUCB-MSCs) and evaluated their potential for chondrocyte differentiation for use in cartilage repair therapy. Our results show that isolated eUCB-MSCs had high proliferative capacity and differentiated easily into osteoblasts and chondrocytes, but not into adipocytes. A three-dimensional (3D) culture approach with the chondrogenic factors BMP-2 and TGF-β1 potentiated chondrogenic differentiation with a significant increase in cartilage-specific markers at the mRNA level (Col2a1, Acan, Snorc) and the protein level (type II and IIB collagen) without an increase in hypertrophic chondrocyte markers (Col10a1 and Mmp13) in normoxia and in hypoxia. However, these chondrogenic factors caused an increase in type I collagen, which can be reduced using small interfering RNA targeting Col1a2. This study provides robust data on MSCs characterization and demonstrates that eUCB-MSCs have a great potential for cartilage tissue engineering.
Keywords: mesenchymal stem cells; umbilical cord blood; horse; cartilage engineering; chondrogenesis; extracellular matrix; RNA interference mesenchymal stem cells; umbilical cord blood; horse; cartilage engineering; chondrogenesis; extracellular matrix; RNA interference

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MDPI and ACS Style

Desancé, M.; Contentin, R.; Bertoni, L.; Gomez-Leduc, T.; Branly, T.; Jacquet, S.; Betsch, J.-M.; Batho, A.; Legendre, F.; Audigié, F.; et al. Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy. Int. J. Mol. Sci. 2018, 19, 537. https://doi.org/10.3390/ijms19020537

AMA Style

Desancé M, Contentin R, Bertoni L, Gomez-Leduc T, Branly T, Jacquet S, Betsch J-M, Batho A, Legendre F, Audigié F, et al. Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy. International Journal of Molecular Sciences. 2018; 19(2):537. https://doi.org/10.3390/ijms19020537

Chicago/Turabian Style

Desancé, Mélanie, Romain Contentin, Lélia Bertoni, Tangni Gomez-Leduc, Thomas Branly, Sandrine Jacquet, Jean-Marc Betsch, Agnès Batho, Florence Legendre, Fabrice Audigié, and et al. 2018. "Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy" International Journal of Molecular Sciences 19, no. 2: 537. https://doi.org/10.3390/ijms19020537

APA Style

Desancé, M., Contentin, R., Bertoni, L., Gomez-Leduc, T., Branly, T., Jacquet, S., Betsch, J.-M., Batho, A., Legendre, F., Audigié, F., Galéra, P., & Demoor, M. (2018). Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy. International Journal of Molecular Sciences, 19(2), 537. https://doi.org/10.3390/ijms19020537

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