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Genome-Wide Analysis of the NF-YB Gene Family in Gossypium hirsutum L. and Characterization of the Role of GhDNF-YB22 in Embryogenesis

1
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
2
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 40070, China
3
Xinjiang Research Base, State Key Laboratory of Cotton Biology, Xinjiang Agricultural University, Urumqi 830052, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(2), 483; https://doi.org/10.3390/ijms19020483
Received: 7 January 2018 / Revised: 1 February 2018 / Accepted: 3 February 2018 / Published: 6 February 2018
(This article belongs to the Section Molecular Plant Sciences)
Members of the NF-YB transcription factor gene family play important roles in diverse processes related to plant growth and development, such as seed development, drought tolerance, and flowering time. However, the function of NF-YB genes in cotton remains unclear. A total of 23, 24, and 50 NF-YB genes were identified in Gossypium arboreum (G. arboreum), Gossypium raimondii (G. raimondii), and G. hirsutum, respectively. A systematic phylogenetic analysis was carried out in G. arboretum, G. raimondii, G. hirsutum, Arabidopsis thaliana, cacao, rice and, sorghum, where the 150 NF-YB genes were divided into five groups (α–ε). Of these groups, α is the largest clade, and γ contains the LEC1 type NF-YB proteins. Syntenic analyses revealed that paralogues of NF-YB genes in G. hirsutum exhibited good collinearity. Owing to segmental duplication within the A sub-genome (At) and D sub-genome (Dt), there was an expanded set of NF-YB genes in G. hirsutum. Furthermore, we investigated the structures of exons, introns, and conserved motifs of NF-YB genes in upland cotton. Most of the NF-YB genes had only one exon, and the genes from the same clade exhibited a similar motif pattern. Expression data show that most NF-YB genes were expressed ubiquitously, and only a few genes were highly expressed in specific tissues, as confirmed by quantitative real-time PCR (qRT-PCR) analysis. The overexpression of GhDNF-YB22 gene, predominantly expressed in embryonic tissues, indicates that GhDNF-YB22 may affect embryogenesis in cotton. This study is the first comprehensive characterization of the GhNF-YB gene family in cotton, and showed that NF-YB genes could be divided into five clades. The duplication events that occurred over the course of evolution were the major impetus for NF-YB gene expansion in upland cotton. Collectively, this work provides insight into the evolution of NF-YB in cotton and further our knowledge of this commercially important species. View Full-Text
Keywords: genome-wide analysis; NF-YB transcription factor; Gossypium hirsutum; overexpression; embryogenesis genome-wide analysis; NF-YB transcription factor; Gossypium hirsutum; overexpression; embryogenesis
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MDPI and ACS Style

Chen, Y.; Yang, Z.; Xiao, Y.; Wang, P.; Wang, Y.; Ge, X.; Zhang, C.; Zhang, X.; Li, F. Genome-Wide Analysis of the NF-YB Gene Family in Gossypium hirsutum L. and Characterization of the Role of GhDNF-YB22 in Embryogenesis. Int. J. Mol. Sci. 2018, 19, 483. https://doi.org/10.3390/ijms19020483

AMA Style

Chen Y, Yang Z, Xiao Y, Wang P, Wang Y, Ge X, Zhang C, Zhang X, Li F. Genome-Wide Analysis of the NF-YB Gene Family in Gossypium hirsutum L. and Characterization of the Role of GhDNF-YB22 in Embryogenesis. International Journal of Molecular Sciences. 2018; 19(2):483. https://doi.org/10.3390/ijms19020483

Chicago/Turabian Style

Chen, Yanli, Zhaoen Yang, Yanqing Xiao, Peng Wang, Ye Wang, Xiaoyang Ge, Chaojun Zhang, Xianlong Zhang, and Fuguang Li. 2018. "Genome-Wide Analysis of the NF-YB Gene Family in Gossypium hirsutum L. and Characterization of the Role of GhDNF-YB22 in Embryogenesis" International Journal of Molecular Sciences 19, no. 2: 483. https://doi.org/10.3390/ijms19020483

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