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Open AccessArticle

Transcriptome and Hormone Comparison of Three Cytoplasmic Male Sterile Systems in Brassica napus

Key Laboratory for Biological Sciences and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
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Int. J. Mol. Sci. 2018, 19(12), 4022; https://doi.org/10.3390/ijms19124022
Received: 1 November 2018 / Revised: 7 December 2018 / Accepted: 11 December 2018 / Published: 12 December 2018
(This article belongs to the Special Issue Plant Genetics and Molecular Breeding)
The interaction between plant mitochondria and the nucleus markedly influences stress responses and morphological features, including growth and development. An important example of this interaction is cytoplasmic male sterility (CMS), which results in plants producing non-functional pollen. In current research work, we compared the phenotypic differences in floral buds of different Brassica napus CMS (Polima, Ogura, Nsa) lines with their corresponding maintainer lines. By comparing anther developmental stages between CMS and maintainer lines, we identified that in the Nsa CMS line abnormality occurred at the tetrad stage of pollen development. Phytohormone assays demonstrated that IAA content decreased in sterile lines as compared to maintainer lines, while the total hormone content was increased two-fold in the S2 stage compared with the S1 stage. ABA content was higher in the S1 stage and exhibited a two-fold decreasing trend in S2 stage. Sterile lines however, had increased ABA content at both stages compared with the corresponding maintainer lines. Through transcriptome sequencing, we compared differentially expressed unigenes in sterile and maintainer lines at both (S1 and S2) developmental stages. We also explored the co-expressed genes of the three sterile lines in the two stages and classified these genes by gene function. By analyzing transcriptome data and validating by RT-PCR, it was shown that some transcription factors (TFs) and hormone-related genes were weakly or not expressed in the sterile lines. This research work provides preliminary identification of the pollen abortion stage in Nsa CMS line. Our focus on genes specifically expressed in sterile lines may be useful to understand the regulation of CMS. View Full-Text
Keywords: cytoplasmic male sterility (CMS); phytohormones; differentially expressed genes; pollen development; Brassica napus cytoplasmic male sterility (CMS); phytohormones; differentially expressed genes; pollen development; Brassica napus
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Ding, B.; Hao, M.; Mei, D.; Zaman, Q.U.; Sang, S.; Wang, H.; Wang, W.; Fu, L.; Cheng, H.; Hu, Q. Transcriptome and Hormone Comparison of Three Cytoplasmic Male Sterile Systems in Brassica napus . Int. J. Mol. Sci. 2018, 19, 4022.

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