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Int. J. Mol. Sci. 2018, 19(12), 3936; https://doi.org/10.3390/ijms19123936

Rice Overexpressing OsNUC1-S Reveals Differential Gene Expression Leading to Yield Loss Reduction after Salt Stress at the Booting Stage

1
Center of Excellence in Environment and Plant Physiology, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
2
Surawiwat School, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
3
Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
4
Department of Plant Biology and Genome Center, University of California Davis, Davis, CA 95616, USA
5
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
6
Omics Science Center, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
*
Author to whom correspondence should be addressed.
Received: 17 November 2018 / Revised: 26 November 2018 / Accepted: 27 November 2018 / Published: 7 December 2018
(This article belongs to the Special Issue Salinity Tolerance in Plants)
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Abstract

Rice nucleolin (OsNUC1), consisting of two isoforms, OsNUC1-L and OsNUC1-S, is a multifunctional protein involved in salt-stress tolerance. Here, OsNUC1-S’s function was investigated using transgenic rice lines overexpressing OsNUC1-S. Under non-stress conditions, the transgenic lines showed a lower yield, but higher net photosynthesis rates, stomatal conductance, and transpiration rates than wild type only in the second leaves, while in the flag leaves, these parameters were similar among the lines. However, under salt-stress conditions at the booting stage, the higher yields in transgenic lines were detected. Moreover, the gas exchange parameters of the transgenic lines were higher in both flag and second leaves, suggesting a role for OsNUC1-S overexpression in photosynthesis adaptation under salt-stress conditions. Moreover, the overexpression lines could maintain light-saturation points under salt-stress conditions, while a decrease in the light-saturation point owing to salt stress was found in wild type. Based on a transcriptome comparison between wild type and a transgenic line, after 3 and 9 days of salt stress, the significantly differentially expressed genes were enriched in the metabolic process of nucleic acid and macromolecule, photosynthesis, water transport, and cellular homeostasis processes, leading to the better performance of photosynthetic processes under salt-stress conditions at the booting stage. View Full-Text
Keywords: RNA binding protein; nucleolin; salt stress; photosynthesis; light saturation point; booting stage; transcriptome RNA binding protein; nucleolin; salt stress; photosynthesis; light saturation point; booting stage; transcriptome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Boonchai, C.; Udomchalothorn, T.; Sripinyowanich, S.; Comai, L.; Buaboocha, T.; Chadchawan, S. Rice Overexpressing OsNUC1-S Reveals Differential Gene Expression Leading to Yield Loss Reduction after Salt Stress at the Booting Stage. Int. J. Mol. Sci. 2018, 19, 3936.

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