Characterization of Heterotrimeric G Protein γ4 Subunit in Rice
AbstractHeterotrimeric G proteins are the molecule switch that transmits information from external signals to intracellular target proteins in mammals and yeast cells. In higher plants, heterotrimeric G proteins regulate plant architecture. Rice harbors one canonical α subunit gene (RGA1), four extra-large GTP-binding protein genes (XLGs), one canonical β-subunit gene (RGB1), and five γ-subunit genes (tentatively designated RGG1, RGG2, RGG3/GS3/Mi/OsGGC1, RGG4/DEP1/DN1/qPE9-1/OsGGC3, and RGG5/OsGGC2) as components of the heterotrimeric G protein complex. Among the five γ-subunit genes, RGG1 encodes the canonical γ-subunit, RGG2 encodes a plant-specific type of γ-subunit with additional amino acid residues at the N-terminus, and the remaining three γ-subunit genes encode atypical γ-subunits with cysteine-rich C-termini. We characterized the RGG4/DEP1/DN1/qPE9-1/OsGGC3 gene product Gγ4 in the wild type (WT) and truncated protein Gγ4∆Cys in the RGG4/DEP1/DN1/qPE9-1/OsGGC3 mutant, Dn1-1, as littele information regarding the native Gγ4 and Gγ4∆Cys proteins is currently available. Based on liquid chromatography-tandem mass spectrometry analysis, immunoprecipitated Gγ4 candidates were confirmed as actual Gγ4. Similar to α-(Gα) and β-subunits (Gβ), Gγ4 was enriched in the plasma membrane fraction and accumulated in the developing leaf sheath. As RGG4/DEP1/DN1/qPE9-1/OsGGC3 mutants exhibited dwarfism, tissues that accumulated Gγ4 corresponded to the abnormal tissues observed in RGG4/DEP1/DN1/qPE9-1/OsGGC3 mutants. View Full-Text
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Matsuta, S.; Nishiyama, A.; Chaya, G.; Itoh, T.; Miura, K.; Iwasaki, Y. Characterization of Heterotrimeric G Protein γ4 Subunit in Rice. Int. J. Mol. Sci. 2018, 19, 3596.
Matsuta S, Nishiyama A, Chaya G, Itoh T, Miura K, Iwasaki Y. Characterization of Heterotrimeric G Protein γ4 Subunit in Rice. International Journal of Molecular Sciences. 2018; 19(11):3596.Chicago/Turabian Style
Matsuta, Sakura; Nishiyama, Aki; Chaya, Genki; Itoh, Takafumi; Miura, Kotaro; Iwasaki, Yukimoto. 2018. "Characterization of Heterotrimeric G Protein γ4 Subunit in Rice." Int. J. Mol. Sci. 19, no. 11: 3596.
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