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Open AccessArticle

DNA Methylation Analysis of Dormancy Release in Almond (Prunus dulcis) Flower Buds Using Epi-Genotyping by Sequencing

Department of Plant Breeding, CEBAS-CSIC, P.O. Box 164, Espinardo, 30100 Murcia, Spain
Department of Plant Biology, Faculty of Biology, University of Murcia, Espinardo, 30100 Murcia, Spain
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(11), 3542;
Received: 24 September 2018 / Revised: 5 November 2018 / Accepted: 7 November 2018 / Published: 10 November 2018
(This article belongs to the Special Issue Plant Genetics and Molecular Breeding)
DNA methylation and histone post-translational modifications have been described as epigenetic regulation mechanisms involved in developmental transitions in plants, including seasonal changes in fruit trees. In species like almond (Prunus dulcis (Mill.) D.A: Webb), prolonged exposure to cold temperatures is required for dormancy release and flowering. Aiming to identify genomic regions with differential methylation states in response to chill accumulation, we carried out Illumina reduced-representation genome sequencing on bisulfite-treated DNA from floral buds. To do this, we analyzed almond genotypes with different chilling requirements and flowering times both before and after dormancy release for two consecutive years. The study was performed using epi-Genotyping by Sequencing (epi-GBS). A total of 7317 fragments were sequenced and the samples compared. Out of these fragments, 677 were identified as differentially methylated between the almond genotypes. Mapping these fragments using the Prunus persica (L.) Batsch v.2 genome as reference provided information about coding regions linked to early and late flowering methylation markers. Additionally, the methylation state of ten gene-coding sequences was found to be linked to the dormancy release process. View Full-Text
Keywords: Prunus; flowering; bisulfite sequencing; genomics; epigenetics; breeding Prunus; flowering; bisulfite sequencing; genomics; epigenetics; breeding
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MDPI and ACS Style

Prudencio, Á.S.; Werner, O.; Martínez-García, P.J.; Dicenta, F.; Ros, R.M.; Martínez-Gómez, P. DNA Methylation Analysis of Dormancy Release in Almond (Prunus dulcis) Flower Buds Using Epi-Genotyping by Sequencing. Int. J. Mol. Sci. 2018, 19, 3542.

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