Endogenous Ovarian Angiogenesis in Polycystic Ovary Syndrome-Like Rats Induced by Low-Frequency Electro-Acupuncture: The CLARITY Three-Dimensional Approach
Abstract
:1. Introduction
2. Results
2.1. Pathological Manifestations in DHT-Induced PCOS-Like Rats and the Effect of EA
2.2. Quantitative Analysis of Follicles and CL in Ovaries Using the CLARITY Methods.
2.3. EA Promotes Angiogenesis in PCOS-Like Ovaries
2.4. EA Promotes Antral Follicle Maturation through Their Surrounding Neovasculature
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Establishment of the PCOS-Like Rat
4.3. Low-Frequency EA
4.4. CLARITY Approach
4.5. Immunofluorescence Staining
4.6. Digital Imaging and Data Analysis
4.7. Data Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
PCOS | Polycystic ovary syndrome |
EA | Electro-acupuncture |
DHT | 5α-dihydrotestosterone |
CL | Corpus luteum |
VEGF | Vascular endothelial growth factor |
PDGF | Platelet derived growth factor |
TGF-β | Transforming growth factor beta |
bFGF | Basic fibroblast growth factor |
PECAM-1/CD31 | Platelet endothelial cell adhesion molecule 1 |
PCNA | Proliferating cell nuclear antigen |
DHEA | dehydroepiandrosterone |
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Ma, T.; Cui, P.; Tong, X.; Hu, W.; Shao, L.R.; Zhang, F.; Li, X.; Feng, Y. Endogenous Ovarian Angiogenesis in Polycystic Ovary Syndrome-Like Rats Induced by Low-Frequency Electro-Acupuncture: The CLARITY Three-Dimensional Approach. Int. J. Mol. Sci. 2018, 19, 3500. https://doi.org/10.3390/ijms19113500
Ma T, Cui P, Tong X, Hu W, Shao LR, Zhang F, Li X, Feng Y. Endogenous Ovarian Angiogenesis in Polycystic Ovary Syndrome-Like Rats Induced by Low-Frequency Electro-Acupuncture: The CLARITY Three-Dimensional Approach. International Journal of Molecular Sciences. 2018; 19(11):3500. https://doi.org/10.3390/ijms19113500
Chicago/Turabian StyleMa, Tong, Peng Cui, Xiaoyu Tong, Wei Hu, Linus R. Shao, Feifei Zhang, Xin Li, and Yi Feng. 2018. "Endogenous Ovarian Angiogenesis in Polycystic Ovary Syndrome-Like Rats Induced by Low-Frequency Electro-Acupuncture: The CLARITY Three-Dimensional Approach" International Journal of Molecular Sciences 19, no. 11: 3500. https://doi.org/10.3390/ijms19113500