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Open AccessArticle

Global Analysis of WOX Transcription Factor Gene Family in Brassica napus Reveals Their Stress- and Hormone-Responsive Patterns

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College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
2
Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(11), 3470; https://doi.org/10.3390/ijms19113470
Received: 28 September 2018 / Revised: 26 October 2018 / Accepted: 30 October 2018 / Published: 5 November 2018
(This article belongs to the Section Molecular Genetics and Genomics)
The plant-specific WUSCHEL-related homeobox (WOX) transcription factor gene family is important for plant growth and development but little studied in oil crops. We identified and characterized 58 putative WOX genes in Brassica napus (BnWOXs), which were divided into three major clades and nine subclades based on the gene structure and conserved motifs. Collinearity analysis revealed that most BnWOXs were the products of allopolyploidization and segmental duplication events. Gene structure analysis indicated that introns/exons and protein motifs were conserved in each subclade and RNA sequencing revealed that BnWOXs had narrow expression profiles in major tissues and/or organs across different developmental stages. The expression pattern of each clade was highly conserved and similar to that of the sister and orthologous pairs from Brassica rapa and Brassica oleracea. Quantitative real-time polymerase chain reaction showed that members of the WOX4 subclade were induced in seedling roots by abiotic and hormone stresses, indicating their contribution to root development and abiotic stress responses. 463 proteins were predicted to interact with BnWOXs, including peptides regulating stem cell homeostasis in meristems. This study provides insights into the evolution and expression of the WOX gene family in B. napus and will be useful in future gene function research. View Full-Text
Keywords: WOX family; Brassica napus L.; phylogenetic analysis; expression analysis WOX family; Brassica napus L.; phylogenetic analysis; expression analysis
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Wang, M.-M.; Liu, M.-M.; Ran, F.; Guo, P.-C.; Ke, Y.-Z.; Wu, Y.-W.; Wen, J.; Li, P.-F.; Li, J.-N.; Du, H. Global Analysis of WOX Transcription Factor Gene Family in Brassica napus Reveals Their Stress- and Hormone-Responsive Patterns. Int. J. Mol. Sci. 2018, 19, 3470.

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