Dietary Sodium Restriction Reduces Arterial Stiffness, Vascular TGF-β-Dependent Fibrosis and Marinobufagenin in Young Normotensive Rats
Abstract
:1. Introduction
2. Results
3. Discussion
4. Methods
4.1. Animal Study
4.2. Pulse Wave Velocity Measurement
4.3. Blood Pressure Measurement
4.4. Immunoassay
4.5. Electrolytes in Plasma and Urine
4.6. Creatinine and FENa
4.7. Histochemistry
4.8. Real-Time Quantitative PCR
4.9. Vascular Smooth Muscle Cell Culture
4.10. Western Blotting Analysis
4.11. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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NS4 (n = 8) | NS8 (n = 8) | HS4 (n = 8) | HS8 (n = 8) | HS4/NS4 (n = 8) | |
---|---|---|---|---|---|
Body weight (g) | 472 ± 7 | 519 ± 13 | 514 ± 30 | 547 ± 17 | 553 ± 18 |
SBP (mmHg) | 126 ± 4 | 128 ± 7 | 125 ± 5 | 126 ± 6 | 121 ± 4 |
Heart rate (beats/min) | 336 ± 37 | 366 ± 8 | 342 ± 6 | 343 ± 9 | 345 ± 11 |
Urine volume (mL/kg BW) | 23.8 ± 0.9 | 44.9 ± 5.1 | 62.8 ± 5.3 * | 64.2 ± 11.6 | 27.6 ± 3.9 † |
Urinary Na (mmol/24 h/kg BW) | 2.9 ± 0.4 | 2.3 ± 0.4 | 30.6 ± 6.6 * | 33.4 ± 3.3 # | 2.8 ± 0.5 † |
Plasma Na (mmol/L) | 141.0 ± 0.7 | 140.0 ± 0.2 | 141.0 ± 0.5 | 141.1 ± 0.5 | 140.2 ± 0.3 |
Plasma K (mmol/L) | 4.5 ± 0.1 | 4.7 ± 0.1 | 4.4 ± 0.1 | 4.8 ± 0.2 | 4.5 ± 0.1 |
Aortic weights (g/mm/kg BW) | 2.44 ± 0.08 | 2.58 ± 0.05 | 2.68 ± 0.12 | 2.86 ± 0.2 | 2.63 ± 0.1 |
NS4 (n = 8) | NS8 (n = 8) | HS4 (n = 8) | HS8 (n = 8) | HS4/NS4 (n = 8) | |
---|---|---|---|---|---|
Urinary Na, mmol/24 h | 1.48 ± 0.21 | 1.29 ± 0.25 | 15.3 ± 3.2 ** | 18.4 ± 2.0 ## | 1.57 ± 0.31 †† |
Plasma Na, mmol/L | 141.0 ± 0.7 | 140.0 ± 0.2 | 141.0 ± 0.5 | 141.1 ± 0.5 | 140.2 ± 0.3 |
Urine creatinine, mg/dL | 196 ± 12 | 100 ± 17 | 76 ± 15 ** | 64 ± 11 | 217 ± 29 †† |
Plasma Creatinine, mg/dL | 0.33 ± 0.02 | 0.38 ± 0.03 | 0.35 ± 0.03 | 0.37 ± 0.02 | 0.37 ± 0.02 |
Urine volume, mL | 11.3 ± 0.4 | 23.0 ± 2.6 | 31.8 ± 2.3 ** | 35.5 ± 6.9 ## | 15 ± 1.9 †† |
Creatinine clearance, mL/min | 4.7 ± 0.4 | 4.1 ± 0.5 | 4.0 ± 0.9 | 4.22 ± 1.1 | 5.2 ± 0.4 |
FeNa, % | 0.08 ± 0.03 | 0.15 ± 0.03 | 2.5 ± 0.51 * | 3.16 ± 1.34 ## | 0.15 ± 0.02 †† |
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Grigorova, Y.N.; Wei, W.; Petrashevskaya, N.; Zernetkina, V.; Juhasz, O.; Fenner, R.; Gilbert, C.; Lakatta, E.G.; Shapiro, J.I.; Bagrov, A.Y.; et al. Dietary Sodium Restriction Reduces Arterial Stiffness, Vascular TGF-β-Dependent Fibrosis and Marinobufagenin in Young Normotensive Rats. Int. J. Mol. Sci. 2018, 19, 3168. https://doi.org/10.3390/ijms19103168
Grigorova YN, Wei W, Petrashevskaya N, Zernetkina V, Juhasz O, Fenner R, Gilbert C, Lakatta EG, Shapiro JI, Bagrov AY, et al. Dietary Sodium Restriction Reduces Arterial Stiffness, Vascular TGF-β-Dependent Fibrosis and Marinobufagenin in Young Normotensive Rats. International Journal of Molecular Sciences. 2018; 19(10):3168. https://doi.org/10.3390/ijms19103168
Chicago/Turabian StyleGrigorova, Yulia N., Wen Wei, Natalia Petrashevskaya, Valentina Zernetkina, Ondrej Juhasz, Rachel Fenner, Christian Gilbert, Edward G. Lakatta, Joseph I. Shapiro, Alexei Y. Bagrov, and et al. 2018. "Dietary Sodium Restriction Reduces Arterial Stiffness, Vascular TGF-β-Dependent Fibrosis and Marinobufagenin in Young Normotensive Rats" International Journal of Molecular Sciences 19, no. 10: 3168. https://doi.org/10.3390/ijms19103168