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Int. J. Mol. Sci. 2018, 19(10), 3120; https://doi.org/10.3390/ijms19103120

Hydrophilic Astragalin Galactoside Induces T Helper Type 1-Mediated Immune Responses via Dendritic Cells

1
Department of Life Sciences, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea
2
Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea
3
Institute of Convergence Science, Korea University, Seoul 02841, Korea
*
Author to whom correspondence should be addressed.
Received: 20 August 2018 / Revised: 22 September 2018 / Accepted: 9 October 2018 / Published: 11 October 2018
(This article belongs to the Special Issue Bioactive Nutrients, Immunity and Inflammation)
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Abstract

A flavonoid Astragalin (kaempferol-3-O-β-d-glucopyranoside, Ast) has several biological activities including anti-oxidant, anti-HIV, and anti-allergic effects. Nonetheless, its insolubility in hydrophilic solvents imposes restrictions on its therapeutic applications. In this study, we investigated the effects of water-soluble astragalin-galactoside (kaempferol-3-O-β-d-isomaltotrioside, Ast-Gal) on murine bone marrow-derived dendritic cell (DC) maturation and T helper (Th) cell-mediated immune responses. Ast-Gal significantly increased maturation and activation of DCs through the upregulation of surface markers, such as cluster of differentiation (CD)80, CD86, and Major histocompatibility complex (MHC) II in a dose-dependent manner, while Ast had little effects. Additionally, Ast-Gal-treated DCs markedly secreted immune-stimulating cytokines such as interleukin (IL)-1β, IL-6, and IL-12. Importantly, Ast-Gal strongly increased expression of IL-12, a polarizing cytokine of Th1 cells. In a co-culture system of DCs and CD4+ T cells, Ast-Gal-treated DCs preferentially differentiates naïve CD4+ T cells into Th1 cells. The addition of neutralizing IL-12 monoclonal antibody (mAb) to cultures of Ast-Gal-treated DCs and CD4+ T cells significantly decreased interferon (IFN)-γ production, thereby indicating that Ast-Gal-stimulated DCs enhance the Th1 response through IL-12 production by DCs. Injection with Ast-Gal-treated DCs in mice increased IFN-γ-secreting Th1 cell population. Collectively, these findings indicate that hydrophilically modified astragalin can enhance Th1-mediated immune responses via DCs and point to a possible application of water-soluble astragalin-galactoside as an immune adjuvant. View Full-Text
Keywords: astragalin galactoside; hydrophilic modification; Th1 cell; dendritic cell; adjuvant astragalin galactoside; hydrophilic modification; Th1 cell; dendritic cell; adjuvant
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Jeon, J.H.; Lee, B.-C.; Kim, D.; Cho, D.; Kim, T.S. Hydrophilic Astragalin Galactoside Induces T Helper Type 1-Mediated Immune Responses via Dendritic Cells. Int. J. Mol. Sci. 2018, 19, 3120.

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