Next Article in Journal
Molecular Features Distinguish Gastric Cancer Subtypes
Next Article in Special Issue
Chlorogenic Acid (CGA) Isomers Alleviate Interleukin 8 (IL-8) Production in Caco-2 Cells by Decreasing Phosphorylation of p38 and Increasing Cell Integrity
Previous Article in Journal
Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation
Previous Article in Special Issue
The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber
 
 
Article

Hydrophilic Astragalin Galactoside Induces T Helper Type 1-Mediated Immune Responses via Dendritic Cells

1
Department of Life Sciences, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea
2
Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea
3
Institute of Convergence Science, Korea University, Seoul 02841, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(10), 3120; https://doi.org/10.3390/ijms19103120
Received: 20 August 2018 / Revised: 22 September 2018 / Accepted: 9 October 2018 / Published: 11 October 2018
(This article belongs to the Special Issue Bioactive Nutrients, Immunity and Inflammation)
A flavonoid Astragalin (kaempferol-3-O-β-d-glucopyranoside, Ast) has several biological activities including anti-oxidant, anti-HIV, and anti-allergic effects. Nonetheless, its insolubility in hydrophilic solvents imposes restrictions on its therapeutic applications. In this study, we investigated the effects of water-soluble astragalin-galactoside (kaempferol-3-O-β-d-isomaltotrioside, Ast-Gal) on murine bone marrow-derived dendritic cell (DC) maturation and T helper (Th) cell-mediated immune responses. Ast-Gal significantly increased maturation and activation of DCs through the upregulation of surface markers, such as cluster of differentiation (CD)80, CD86, and Major histocompatibility complex (MHC) II in a dose-dependent manner, while Ast had little effects. Additionally, Ast-Gal-treated DCs markedly secreted immune-stimulating cytokines such as interleukin (IL)-1β, IL-6, and IL-12. Importantly, Ast-Gal strongly increased expression of IL-12, a polarizing cytokine of Th1 cells. In a co-culture system of DCs and CD4+ T cells, Ast-Gal-treated DCs preferentially differentiates naïve CD4+ T cells into Th1 cells. The addition of neutralizing IL-12 monoclonal antibody (mAb) to cultures of Ast-Gal-treated DCs and CD4+ T cells significantly decreased interferon (IFN)-γ production, thereby indicating that Ast-Gal-stimulated DCs enhance the Th1 response through IL-12 production by DCs. Injection with Ast-Gal-treated DCs in mice increased IFN-γ-secreting Th1 cell population. Collectively, these findings indicate that hydrophilically modified astragalin can enhance Th1-mediated immune responses via DCs and point to a possible application of water-soluble astragalin-galactoside as an immune adjuvant. View Full-Text
Keywords: astragalin galactoside; hydrophilic modification; Th1 cell; dendritic cell; adjuvant astragalin galactoside; hydrophilic modification; Th1 cell; dendritic cell; adjuvant
Show Figures

Figure 1

MDPI and ACS Style

Jeon, J.H.; Lee, B.-C.; Kim, D.; Cho, D.; Kim, T.S. Hydrophilic Astragalin Galactoside Induces T Helper Type 1-Mediated Immune Responses via Dendritic Cells. Int. J. Mol. Sci. 2018, 19, 3120. https://doi.org/10.3390/ijms19103120

AMA Style

Jeon JH, Lee B-C, Kim D, Cho D, Kim TS. Hydrophilic Astragalin Galactoside Induces T Helper Type 1-Mediated Immune Responses via Dendritic Cells. International Journal of Molecular Sciences. 2018; 19(10):3120. https://doi.org/10.3390/ijms19103120

Chicago/Turabian Style

Jeon, Jae Hyoung, Byung-Cheol Lee, Doman Kim, Daeho Cho, and Tae Sung Kim. 2018. "Hydrophilic Astragalin Galactoside Induces T Helper Type 1-Mediated Immune Responses via Dendritic Cells" International Journal of Molecular Sciences 19, no. 10: 3120. https://doi.org/10.3390/ijms19103120

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop