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Article

Genome-Wide Association Analysis of Mucilage and Hull Content in Flax (Linum usitatissimum L.) Seeds

1
Agriaquaculture Nutritional Genomic Center (CGNA), Las Heras 350, Temuco 4781158, Chile
2
Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada
3
Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB R6M 1Y5, Canada
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(10), 2870; https://doi.org/10.3390/ijms19102870
Received: 14 August 2018 / Revised: 14 September 2018 / Accepted: 18 September 2018 / Published: 21 September 2018
(This article belongs to the Special Issue Plant Genomics)
New flaxseed cultivars differing in seed mucilage content (MC) with low hull content (HC) represent an attractive option to simultaneously target the food and feed markets. Here, a genome-wide association study (GWAS) was conducted for MC and HC in 200 diverse flaxseed accessions genotyped with 1.7 million single nucleotide polymorphism (SNP) markers. The data obtained for MC and HC indicated a broad phenotypic variation and high (~70%) and a moderate (~49%) narrow sense heritability, respectively. MC and HC did not differ statistically between fiber and oil morphotypes, but yellow-seeded accessions had 2.7% less HC than brown-seeded ones. The genome-wide linkage disequilibrium (LD) decayed to r2 = 0.1 at a physical distance of ~100 kb. Seven and four quantitative trait loci (QTL) were identified for MC and HC, respectively. Promising candidate genes identified include Linum usitatissimum orthologs of the Arabidopsis thaliana genes TRANSPARENT TESTA 8, SUBTILISIN-LIKE SERINE PROTEASE, GALACTUROSYL TRANSFERASE-LIKE 5, MUCILAGE-MODIFIED 4, AGAMOUS-LIKE MADS-BOX PROTEIN AGL62, GLYCOSYL HYDROLASE FAMILY 17, and UDP-GLUCOSE FLAVONOL 3-O-GLUCOSYLTRANSFERASE. These genes have been shown to play a role in mucilage synthesis and release, seed coat development and anthocyanin biosynthesis in A. thaliana. The favorable alleles will be useful in flaxseed breeding towards the goal of achieving the ideal MC and HC composition for food and feed by genomic-based breeding. View Full-Text
Keywords: flaxseed; Linum usitatissimum; GWAS; seed mucilage content; seed hull content; single nucleotide polymorphism (SNP) flaxseed; Linum usitatissimum; GWAS; seed mucilage content; seed hull content; single nucleotide polymorphism (SNP)
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MDPI and ACS Style

Soto-Cerda, B.J.; Cloutier, S.; Quian, R.; Gajardo, H.A.; Olivos, M.; You, F.M. Genome-Wide Association Analysis of Mucilage and Hull Content in Flax (Linum usitatissimum L.) Seeds. Int. J. Mol. Sci. 2018, 19, 2870. https://doi.org/10.3390/ijms19102870

AMA Style

Soto-Cerda BJ, Cloutier S, Quian R, Gajardo HA, Olivos M, You FM. Genome-Wide Association Analysis of Mucilage and Hull Content in Flax (Linum usitatissimum L.) Seeds. International Journal of Molecular Sciences. 2018; 19(10):2870. https://doi.org/10.3390/ijms19102870

Chicago/Turabian Style

Soto-Cerda, Braulio J., Sylvie Cloutier, Rocío Quian, Humberto A. Gajardo, Marcos Olivos, and Frank M. You. 2018. "Genome-Wide Association Analysis of Mucilage and Hull Content in Flax (Linum usitatissimum L.) Seeds" International Journal of Molecular Sciences 19, no. 10: 2870. https://doi.org/10.3390/ijms19102870

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