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Int. J. Mol. Sci. 2018, 19(1), 37;

Detection of Plant miRNAs Abundance in Human Breast Milk

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland
Department of Plant Molecular Biology, Institute of Experimental Plant Biology and Biotechnology, University of Warsaw, 02-096 Warsaw, Poland
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 19 November 2017 / Revised: 12 December 2017 / Accepted: 21 December 2017 / Published: 23 December 2017
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [1455 KB, uploaded 23 December 2017]   |  


Breast milk is a natural food and important component of infant nutrition. Apart from the alimentary substances, breast milk contains many important bioactive compounds, including endogenous microRNA molecules (miRNAs). These regulatory molecules were identified in various mammalian biological fluids and were shown to be mostly packed in exosomes. Recently, it was revealed that plant food-derived miRNAs are stably present in human blood and regulate the expression of specific human genes. Since then, the scientific community has focused its efforts on contradicting or confirming this discovery. With the same intention, qRT-PCR experiments were performed to evaluate the presence of five plant food-derived miRNAs (miR166a, miR156a, miR157a, miR172a and miR168a) in breast milk (whole milk and exosomes) from healthy volunteers. In whole milk samples, all examined miRNAs were identified, while only two of these miRNAs were confirmed to be present in exosomes. The plant miRNA concentration in the samples ranged from 4 to 700 fM. Complementary bioinformatics analysis suggests that the evaluated plant miRNAs may potentially influence several crucial biological pathways in the infant organism. View Full-Text
Keywords: plant miRNA; human breast milk; exosomes; cross-kingdom; gene expression regulation plant miRNA; human breast milk; exosomes; cross-kingdom; gene expression regulation

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Lukasik, A.; Brzozowska, I.; Zielenkiewicz, U.; Zielenkiewicz, P. Detection of Plant miRNAs Abundance in Human Breast Milk. Int. J. Mol. Sci. 2018, 19, 37.

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