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Int. J. Mol. Sci. 2018, 19(1), 194;

Calbindin D28k-Immunoreactivity in Human Enteric Neurons

Institute of Anatomy and Cell Biology, University of Erlangen-Nuremberg, Krankenhausstraße 9, D-91054 Erlangen, Germany
Institute of Pathology, University of Erlangen-Nuremberg, Krankenhausstraße 8-10, D-91054 Erlangen, Germany
Author to whom correspondence should be addressed.
Received: 18 December 2017 / Revised: 28 December 2017 / Accepted: 4 January 2018 / Published: 8 January 2018
(This article belongs to the Special Issue Calcium Binding Proteins)
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Calbindin (CALB) is well established as immunohistochemical marker for intrinsic primary afferent neurons in the guinea pig gut. Its expression by numerous human enteric neurons has been demonstrated but little is known about particular types of neurons immunoreactive for CALB. Here we investigated small and large intestinal wholemount sets of 26 tumor patients in order to evaluate (1) the proportion of CALB+ neurons in the total neuron population, (2) the colocalization of CALB with calretinin (CALR), somatostatin (SOM) and vasoactive intestinal peptide (VIP) and (3) the morphology of CALB+ neurons. CALB+ neurons represented a minority of myenteric neurons (small intestine: 31%; large intestine: 25%) and the majority of submucosal neurons (between 72 and 95%). In the submucosa, most CALB+ neurons co-stained for CALR and VIP (between 69 and 80%) or for SOM (between 20 and 3%). In the myenteric plexus, 85% of CALB+ neurons did not co-stain with the other markers investigated. An unequivocal correlation between CALB reactivity and neuronal morphology was found for myenteric type III neurons in the small intestine: uniaxonal neurons with long, slender and branched dendrites were generally positive for CALB. Since also other neurons displayed occasional CALB reactivity, this protein is not suited as an exclusive marker for type III neurons. View Full-Text
Keywords: calcium binding protein; calretinin; enteric nervous system; morphology; myenteric plexus; submucosal plexus calcium binding protein; calretinin; enteric nervous system; morphology; myenteric plexus; submucosal plexus

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zetzmann, K.; Strehl, J.; Geppert, C.; Kuerten, S.; Jabari, S.; Brehmer, A. Calbindin D28k-Immunoreactivity in Human Enteric Neurons. Int. J. Mol. Sci. 2018, 19, 194.

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