Next Article in Journal
Cloning and Functional Analysis of Phosphoethanolamine Methyltransferase Promoter from Maize (Zea mays L.)
Next Article in Special Issue
Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements Facilitates Analysis of Biological Endpoint Effects of Cellular Ca2+ Mobilization
Previous Article in Journal
Scanning the Immunopathogenesis of Psoriasis
Open AccessArticle

Calbindin D28k-Immunoreactivity in Human Enteric Neurons

Institute of Anatomy and Cell Biology, University of Erlangen-Nuremberg, Krankenhausstraße 9, D-91054 Erlangen, Germany
Institute of Pathology, University of Erlangen-Nuremberg, Krankenhausstraße 8-10, D-91054 Erlangen, Germany
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(1), 194;
Received: 18 December 2017 / Revised: 28 December 2017 / Accepted: 4 January 2018 / Published: 8 January 2018
(This article belongs to the Special Issue Calcium Binding Proteins)
Calbindin (CALB) is well established as immunohistochemical marker for intrinsic primary afferent neurons in the guinea pig gut. Its expression by numerous human enteric neurons has been demonstrated but little is known about particular types of neurons immunoreactive for CALB. Here we investigated small and large intestinal wholemount sets of 26 tumor patients in order to evaluate (1) the proportion of CALB+ neurons in the total neuron population, (2) the colocalization of CALB with calretinin (CALR), somatostatin (SOM) and vasoactive intestinal peptide (VIP) and (3) the morphology of CALB+ neurons. CALB+ neurons represented a minority of myenteric neurons (small intestine: 31%; large intestine: 25%) and the majority of submucosal neurons (between 72 and 95%). In the submucosa, most CALB+ neurons co-stained for CALR and VIP (between 69 and 80%) or for SOM (between 20 and 3%). In the myenteric plexus, 85% of CALB+ neurons did not co-stain with the other markers investigated. An unequivocal correlation between CALB reactivity and neuronal morphology was found for myenteric type III neurons in the small intestine: uniaxonal neurons with long, slender and branched dendrites were generally positive for CALB. Since also other neurons displayed occasional CALB reactivity, this protein is not suited as an exclusive marker for type III neurons. View Full-Text
Keywords: calcium binding protein; calretinin; enteric nervous system; morphology; myenteric plexus; submucosal plexus calcium binding protein; calretinin; enteric nervous system; morphology; myenteric plexus; submucosal plexus
Show Figures

Graphical abstract

MDPI and ACS Style

Zetzmann, K.; Strehl, J.; Geppert, C.; Kuerten, S.; Jabari, S.; Brehmer, A. Calbindin D28k-Immunoreactivity in Human Enteric Neurons. Int. J. Mol. Sci. 2018, 19, 194.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop