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Int. J. Mol. Sci. 2018, 19(1), 118; https://doi.org/10.3390/ijms19010118

Seed Transcriptomics Analysis in Camellia oleifera Uncovers Genes Associated with Oil Content and Fatty Acid Composition

1
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China
2
Research Institute of Horticulture, Hunan Academy of Agricultural Sciences, Changsha 410125, China
3
Forestry Seedling Management Station of Zhejiang Province, Hangzhou 310020, China
These authors have contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 2 November 2017 / Revised: 14 December 2017 / Accepted: 27 December 2017 / Published: 2 January 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Camellia oleifera is a major tree species for producing edible oil. Its seed oil is well known for the high level of oleic acids; however, little is known regarding the molecular mechanism of lipid biosynthesis in C. oleifera. Here, we measured the oil contents and fatty acid (FA) compositions at four developmental stages and investigated the global gene expression profiles through transcriptomics sequencing. We identified differentially-expressed genes (DEGs) among the developmental stages and found that the distribution of numbers of DEGs was associated with the accumulation pattern of seed oil. Gene Ontology (GO) enrichment analysis revealed some critical biological processes related to oil accumulation, including lipid metabolism and phosphatidylcholine metabolism. Furthermore, we investigated the expression patterns of lipid biosynthesis genes. We showed that most of the genes were identified with single or multiple copies, and some had correlated profiles along oil accumulation. We proposed that the higher levels of stearoyl-ACP desaturases (SADs) coupled with lower activities of fatty acid desaturase 2 (FAD2) might be responsive to the boost of oleic acid at the late stage of C. oleifera seeds’ development. This work presents a comprehensive transcriptomics study of C. oleifera seeds and uncovers valuable DEGs that are associated with the seed oil accumulation. View Full-Text
Keywords: Camellia oleifera; transcriptomics; lipid biosynthesis; fatty acid; seed oil Camellia oleifera; transcriptomics; lipid biosynthesis; fatty acid; seed oil
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Lin, P.; Wang, K.; Zhou, C.; Xie, Y.; Yao, X.; Yin, H. Seed Transcriptomics Analysis in Camellia oleifera Uncovers Genes Associated with Oil Content and Fatty Acid Composition. Int. J. Mol. Sci. 2018, 19, 118.

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