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Int. J. Mol. Sci. 2017, 18(8), 1778;

Biophysical and Computational Studies of the vCCI:vMIP-II Complex

Departments of Molecular Cell Biology and Chemistry and Chemical Biology, and the Health Sciences Research Institute, University of California Merced 5200 North Lake Rd, Merced, CA 953402, USA
Department of Chemistry & Biochemistry, University of Missouri-St. Louis, St. Louis, MO 63121, USA
Author to whom correspondence should be addressed.
Received: 20 July 2017 / Revised: 8 August 2017 / Accepted: 9 August 2017 / Published: 16 August 2017
(This article belongs to the Special Issue Regulation of Chemokine-Receptor Interactions and Functions)
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Certain viruses have the ability to subvert the mammalian immune response, including interference in the chemokine system. Poxviruses produce the chemokine binding protein vCCI (viral CC chemokine inhibitor; also called 35K), which tightly binds to CC chemokines. To facilitate the study of vCCI, we first provide a protocol to produce folded vCCI from Escherichia coli (E. coli.) It is shown here that vCCI binds with unusually high affinity to viral Macrophage Inflammatory Protein-II (vMIP-II), a chemokine analog produced by the virus, human herpesvirus 8 (HHV-8). Fluorescence anisotropy was used to investigate the vCCI:vMIP-II complex and shows that vCCI binds to vMIP-II with a higher affinity than most other chemokines, having a Kd of 0.06 ± 0.006 nM. Nuclear magnetic resonance (NMR) chemical shift perturbation experiments indicate that key amino acids used for binding in the complex are similar to those found in previous work. Molecular dynamics were then used to compare the vCCI:vMIP-II complex with the known vCCI:Macrophage Inflammatory Protein-1β/CC-Chemokine Ligand 4 (MIP-1β/CCL4) complex. The simulations show key interactions, such as those between E143 and D75 in vCCI/35K and R18 in vMIP-II. Further, in a comparison of 1 μs molecular dynamics (MD) trajectories, vMIP-II shows more overall surface binding to vCCI than does the chemokine MIP-1β. vMIP-II maintains unique contacts at its N-terminus to vCCI that are not made by MIP-1β, and vMIP-II also makes more contacts with the vCCI flexible acidic loop (located between the second and third beta strands) than does MIP-1β. These studies provide evidence for the basis of the tight vCCI:vMIP-II interaction while elucidating the vCCI:MIP-1β interaction, and allow insight into the structure of proteins that are capable of broadly subverting the mammalian immune system. View Full-Text
Keywords: chemokine binding protein; chemokine analog; anti-inflammation; 35K; vCCI; vMIP-II; MIP-1β/CCL4; molecular dynamics chemokine binding protein; chemokine analog; anti-inflammation; 35K; vCCI; vMIP-II; MIP-1β/CCL4; molecular dynamics

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Nguyen, A.F.; Kuo, N.-W.; Showalter, L.J.; Ramos, R.; Dupureur, C.M.; Colvin, M.E.; LiWang, P.J. Biophysical and Computational Studies of the vCCI:vMIP-II Complex. Int. J. Mol. Sci. 2017, 18, 1778.

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