Specific MicroRNA Pattern in Colon Tissue of Young Children with Eosinophilic Colitis
Abstract
:1. Introduction
2. Results
2.1. EC-Specific miR Expression Profile by Next-Generation Sequencing (NGS)
2.2. Expression of miRs Selected for Further Analysis by RT-PCR
2.3. Correlation of miR Expression with Tissue Eosinophil Counts
2.4. Results of Bioinformatics Analysis
3. Discussion
4. Materials and Methods
4.1. Patients
4.2. RNA Isolation
4.3. cDNA Library Preparation and NGS
4.4. RT-PCR
4.5. Bioinformatics Analysis of the Whole NGS miR Profile
4.6. Determination of Eosinophil Counts in the Colonic Tissue of EC Patients
4.7. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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A | Control | Eosinophilic Colitis |
n | 4 | 4 |
Male/Female | 2/2 | 3/1 |
Age (months) | 136.5 ± 35.78 | 8.88 ± 5.14 * |
BMI (kg/m2) | - | 18.66 ± 3.79 |
Iron (µmol/L) | 10.75 ± 2.5 | 13.25 ± 3.57 |
Albumin (g/L) | 51.75 ± 8.81 | - |
Haemoglobin (g/L) | 134.3 ± 7.76 | 121.8 ± 3.64 |
Haematocrit (%) | 0.39 ± 0.02 | 0.34 ± 0.013 |
Platelet count (Giga/L) | 325.8 ± 21.10 | 440.5 ± 57.81 |
CRP (mg/L) | 1.25 ± 1.4 | 0 |
B | Control | Eosinophilic Colitis |
n | 14 | 10 |
Male/Female | 6/8 | 8/2 |
Age (months) | 29.82 ± 17.08 | 5.7 ± 4.76 *** |
BMI (kg/m2) | 16.15 ± 1.6 | 16.21 ± 1.74 |
Length-weight percentile (%) | - | 42.25 ± 34.92 1/10 overweight 1/10 underweight |
Iron (µmol/L) | 15.70 ± 4.9 | 9.89 ± 5.62 * |
Albumin (g/L) | 45.8 ± 2.01 | 44 ± 4.24 |
Haemoglobin (g/L) | 122.1 ± 12.26 | 112.6 ± 8.32 * |
Haematocrit (%) | 35.18 ± 2.8 | 32 ± 1.94 |
Platelet count (Giga/L) | 364.6 ± 87.09 | 394.5 ± 104.1 |
CRP (mg/L) | 0.79 ± 1.4 | 3.44 ± 8.49 |
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Kiss, Z.; Béres, N.J.; Sziksz, E.; Tél, B.; Borka, K.; Arató, A.; Szabó, A.J.; Veres, G. Specific MicroRNA Pattern in Colon Tissue of Young Children with Eosinophilic Colitis. Int. J. Mol. Sci. 2017, 18, 1050. https://doi.org/10.3390/ijms18051050
Kiss Z, Béres NJ, Sziksz E, Tél B, Borka K, Arató A, Szabó AJ, Veres G. Specific MicroRNA Pattern in Colon Tissue of Young Children with Eosinophilic Colitis. International Journal of Molecular Sciences. 2017; 18(5):1050. https://doi.org/10.3390/ijms18051050
Chicago/Turabian StyleKiss, Zoltán, Nóra Judit Béres, Erna Sziksz, Bálint Tél, Katalin Borka, András Arató, Attila J. Szabó, and Gábor Veres. 2017. "Specific MicroRNA Pattern in Colon Tissue of Young Children with Eosinophilic Colitis" International Journal of Molecular Sciences 18, no. 5: 1050. https://doi.org/10.3390/ijms18051050