Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death
Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea
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Author to whom correspondence should be addressed.
Academic Editors: José L. Quiles and Maurizio Battino
Int. J. Mol. Sci. 2017, 18(5), 1012; https://doi.org/10.3390/ijms18051012
Received: 24 January 2017
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Revised: 21 April 2017
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Accepted: 3 May 2017
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Published: 8 May 2017
(This article belongs to the Section Bioactives and Nutraceuticals)
Oxidative stress and apoptosis are the major mechanisms that induce dopaminergic cell death. Our study investigates the protective effects of atractylenolide-I (ATR-I) on 1-methyl-4-phenylpyridinium (MPP+)-induced cytotoxicity in human dopaminergic SH-SY5Y cells, as well as its underlying mechanism. Our experimental data indicates that ATR-I significantly inhibits the loss of cell viability induced by MPP+ in SH-SY5Y cells. To further unravel the mechanism, we examined the effect of ATR-I on MPP+-induced apoptotic cell death characterized by an increase in the Bax/Bcl-2 mRNA ratio, the release of cytochrome-c, and the activation of caspase-3 leading to elevated levels of cleaved poly(ADP-ribose) polymerase (PARP) resulting in SH-SY5Y cell death. Our results demonstrated that ATR-I decreases the level of pro-apoptotic proteins induced by MPP+ and also restored Bax/Bcl-2 mRNA levels, which are critical for inducing apoptosis. In addition, ATR-I demonstrated a significant increase in the protein expression of heme-oxygenase in MPP+-treated SH-SY5Y cells. These results suggest that the pharmacological effect of ATR-I may be, at least in part, caused by the reduction in pro-apoptotic signals and also by induction of anti-oxidant protein.
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Keywords:
atractylenolide-I; apoptosis; MPP+; neuroprotection; Parkinson’s disease
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MDPI and ACS Style
More, S.V.; Choi, D.-K. Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death. Int. J. Mol. Sci. 2017, 18, 1012. https://doi.org/10.3390/ijms18051012
AMA Style
More SV, Choi D-K. Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death. International Journal of Molecular Sciences. 2017; 18(5):1012. https://doi.org/10.3390/ijms18051012
Chicago/Turabian StyleMore, Sandeep V., and Dong-Kug Choi. 2017. "Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death" International Journal of Molecular Sciences 18, no. 5: 1012. https://doi.org/10.3390/ijms18051012
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