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Int. J. Mol. Sci. 2017, 18(3), 520;

Ovalbumin with Glycated Carboxyl Groups Shows Membrane-Damaging Activity

Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Author to whom correspondence should be addressed.
Academic Editor: Masatoshi Maki
Received: 12 January 2017 / Revised: 17 February 2017 / Accepted: 22 February 2017 / Published: 28 February 2017
(This article belongs to the Section Biochemistry)
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The aim of the present study was to investigate whether glycated ovalbumin (OVA) showed novel activity at the lipid-water interface. Mannosylated OVA (Man-OVA) was prepared by modification of the carboxyl groups with p-aminophenyl α-dextro (d)-mannopyranoside. An increase in the number of modified carboxyl groups increased the membrane-damaging activity of Man-OVA on cell membrane-mimicking vesicles, whereas OVA did not induce membrane permeability in the tested phospholipid vesicles. The glycation of carboxyl groups caused a notable change in the gross conformation of OVA. Moreover, owing to their spatial positions, the Trp residues in Man-OVA were more exposed, unlike those in OVA. Fluorescence quenching studies suggested that the Trp residues in Man-OVA were located on the interface binds with the lipid vesicles, and their microenvironment was abundant in positively charged residues. Although OVA and Man-OVA showed a similar binding affinity for lipid vesicles, the lipid-interacting feature of Man-OVA was distinct from that of OVA. Chemical modification studies revealed that Lys and Arg residues, but not Trp residues, played a crucial role in the membrane-damaging activity of Man-OVA. Taken together, our data suggest that glycation of carboxyl groups causes changes in the structural properties and membrane-interacting features of OVA, generating OVA with membrane-perturbing activities at the lipid-water interface. View Full-Text
Keywords: ovalbumin; glycation; carboxyl group; membrane-damaging activity ovalbumin; glycation; carboxyl group; membrane-damaging activity

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Tang, C.-C.; Shi, Y.-J.; Chen, Y.-J.; Chang, L.-S. Ovalbumin with Glycated Carboxyl Groups Shows Membrane-Damaging Activity. Int. J. Mol. Sci. 2017, 18, 520.

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