Evaluation of Not-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems
Pietro Gentile 1,2,*
, John P. Cole 3, Megan A. Cole 3, Simone Garcovich 4, Alessandra Bielli 5, Maria Giovanna Scioli 5, Augusto Orlandi 5, Chiara Insalaco 1,3 and Valerio Cervelli 1
, John P. Cole 3, Megan A. Cole 3, Simone Garcovich 4, Alessandra Bielli 5, Maria Giovanna Scioli 5, Augusto Orlandi 5, Chiara Insalaco 1,3 and Valerio Cervelli 1
1
Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, Via Courmayeur, No. 102, 00135 Rome, Italy
2
Plastic and Reconstructive Surgery Department, Catholic University, 1005 Tiranna, Albania
3
Cole Hair Transplant Group, Alpharetta, 30004 GA, USA
4
Institute of Dermatology, Catholic University of the Sacred Heart, 00168 Rome, Italy
5
Institute of Anatomic Pathology, University of Rome Tor Vergata, 00133 Rome, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Terrence Piva
Int. J. Mol. Sci. 2017, 18(2), 408; https://doi.org/10.3390/ijms18020408
Received: 16 January 2017 / Revised: 7 February 2017 / Accepted: 8 February 2017 / Published: 14 February 2017
(This article belongs to the Section Biochemistry)
Abstract
Platelet rich plasma (PRP) was tested as a potential therapy for androgenetic alopecia (AGA) through two different clinical protocols in which one population (18 participants) received half-head treatment with autologous non-activated PRP (A-PRP) produced by CPunT Preparation System (Biomed Device, Modena, Italy) and the other half-head with placebo, and a second separated population in which all participants (n = 6, 3 participants per group) received treatment with calcium-activated PRP (AA-PRP) produced from one of two different PRP collection devices (Regen Blood Cell Therapy or Arthrex Angel System). For the A-PRP study, three treatments were administered over 30-day intervals. Trichoscan analysis of patients, three months post-treatment, showed a clinical improvement in the number of hairs in the target area (36 ± 3 hairs) and in total hair density (65± 5 hair cm2), whereas negligible improvements in hair count (1.1± 1.4 hairs) and density (1.9 ± 10.2 hair cm2) were seen in the region of the scalp that received placebo. Microscopic evaluation conducted two weeks after treatment showed also an increase in epidermal thickness, Ki67+ keratinocytes, and in the number of follicles. The AA-PRP treatment groups received a singular set of injections, and six months after the treatments were administered, notable differences in clinical outcomes were obtained from the two PRP collection devices (+90 ± 6 hair cm2 versus -73 ± 30 hair cm2 hair densities, Regen versus Arthrex). Growth factor concentrations in AA-PRP prepared from the two collection devices did not differ significantly upon calcium activation. View Full-TextKeywords:
androgenic alopecia; hair loss; autologous PRP; activated PRP; growth factors
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Gentile, P.; Cole, J.P.; Cole, M.A.; Garcovich, S.; Bielli, A.; Scioli, M.G.; Orlandi, A.; Insalaco, C.; Cervelli, V. Evaluation of Not-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems. Int. J. Mol. Sci. 2017, 18, 408.
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