Next Article in Journal
Potent Inhibition of miR-34b on Migration and Invasion in Metastatic Prostate Cancer Cells by Regulating the TGF-β Pathway
Next Article in Special Issue
The Role of Sugar Transporter Genes during Early Infection by Root-Knot Nematodes
Previous Article in Journal
Adjunctive Therapy Approaches for Ischemic Stroke: Innovations to Expand Time Window of Treatment
Previous Article in Special Issue
Overexpression of a New Chitinase Gene EuCHIT2 Enhances Resistance to Erysiphe cichoracearum DC. in Tobacco Plants
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(12), 2765;

Overexpression and Down-Regulation of Barley Lipoxygenase LOX2.2 Affects Jasmonate-Regulated Genes and Aphid Fecundity

Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden
Department of Crop Production Ecology, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden
Department of Plant Breeding, Swedish University of Agricultural Sciences, 23053 Alnarp, Sweden
Cell and Molecular Science, James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 2 November 2017 / Revised: 8 December 2017 / Accepted: 15 December 2017 / Published: 19 December 2017
(This article belongs to the Special Issue Plant Defense Genes Against Biotic Stresses)
Full-Text   |   PDF [1234 KB, uploaded 19 December 2017]   |  


Aphids are pests on many crops and depend on plant phloem sap as their food source. In an attempt to find factors improving plant resistance against aphids, we studied the effects of overexpression and down-regulation of the lipoxygenase gene LOX2.2 in barley (Hordeum vulgare L.) on the performance of two aphid species. A specialist, bird cherry-oat aphid (Rhopalosiphum padi L.) and a generalist, green peach aphid (Myzus persicae Sulzer) were studied. LOX2.2 overexpressing lines showed up-regulation of some other jasmonic acid (JA)-regulated genes, and antisense lines showed down-regulation of such genes. Overexpression or suppression of LOX2.2 did not affect aphid settling or the life span on the plants, but in short term fecundity tests, overexpressing plants supported lower aphid numbers and antisense plants higher aphid numbers. The amounts and composition of released volatile organic compounds did not differ between control and LOX2.2 overexpressing lines. Up-regulation of genes was similar for both aphid species. The results suggest that LOX2.2 plays a role in the activation of JA-mediated responses and indicates the involvement of LOX2.2 in basic defense responses. View Full-Text
Keywords: aphid resistance; lipoxygenase; Hordeum vulgare; Rhopalosiphum padi; Myzus persicae aphid resistance; lipoxygenase; Hordeum vulgare; Rhopalosiphum padi; Myzus persicae

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Losvik, A.; Beste, L.; Glinwood, R.; Ivarson, E.; Stephens, J.; Zhu, L.-H.; Jonsson, L. Overexpression and Down-Regulation of Barley Lipoxygenase LOX2.2 Affects Jasmonate-Regulated Genes and Aphid Fecundity. Int. J. Mol. Sci. 2017, 18, 2765.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top