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Int. J. Mol. Sci. 2017, 18(12), 2710; https://doi.org/10.3390/ijms18122710

High-Throughput RNA-Seq Data Analysis of the Single Nucleotide Polymorphisms (SNPs) and Zygomorphic Flower Development in Pea (Pisum sativum L.)

1,†
,
2,†,* , 1
,
1
and
1
1
Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
2
College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing 210014, China
These authors have contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 24 November 2017 / Revised: 10 December 2017 / Accepted: 12 December 2017 / Published: 20 December 2017
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Pea (Pisum sativum L.) is a model plant that has been used in classical genetics and organ development studies. However, its large and complex genome has hindered research investigations in pea. Here, we generated transcriptomes from different tissues or organs of three pea accessions using next-generation sequencing to assess single nucleotide polymorphisms (SNPs), and further investigated petal differentially expressed genes to elucidate the mechanisms regulating floral zygomorphy. Eighteen samples were sequenced, which yielded a total of 617 million clean reads, and de novo assembly resulted in 87,137 unigenes. A total of 9044 high-quality SNPs were obtained among the three accessions, and a consensus map was constructed. We further discovered several dorsoventral asymmetrically expressed genes that were confirmed by qRT-PCR among different petals, including previously reported three CYC-like proliferating cell factor (TCP) genes. One MADS-box gene was highly expressed in dorsal petals, and several MYB factors were predominantly expressed among dorsal, lateral, and/or ventral petals, together with a ventrally expressed TCP gene. In sum, our comprehensive database complements the existing resources for comparative genetic mapping and facilitates future investigations in legume zygomorphic flower development. View Full-Text
Keywords: pea (Pisum sativum L.); next-generation sequencing; de novo assembly; zygomorphic flower development; dorsoventral asymmetry; petal-specific gene expression pea (Pisum sativum L.); next-generation sequencing; de novo assembly; zygomorphic flower development; dorsoventral asymmetry; petal-specific gene expression
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jiao, K.; Li, X.; Guo, W.; Su, S.; Luo, D. High-Throughput RNA-Seq Data Analysis of the Single Nucleotide Polymorphisms (SNPs) and Zygomorphic Flower Development in Pea (Pisum sativum L.). Int. J. Mol. Sci. 2017, 18, 2710.

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