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Int. J. Mol. Sci. 2017, 18(12), 2696;

Clodronate as a Therapeutic Strategy against Osteoarthritis

Internal Medicine, Section D, Department of Medicine, University of Verona, 37134 Verona, Italy
Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy
Biocrystallography Lab, Department of Biotechnology, University of Verona, 37134 Verona, Italy
Author to whom correspondence should be addressed.
Received: 16 November 2017 / Revised: 4 December 2017 / Accepted: 8 December 2017 / Published: 13 December 2017
(This article belongs to the Special Issue Research of Pathogenesis and Novel Therapeutics in Arthritis)
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Osteoarthritis (OA), the most prevalent musculoskeletal pathology, is mainly characterized by the progressive degradation of articular cartilage due to an imbalance between anabolic and catabolic processes. Consequently, OA has been associated with defects in the chondrocitic differentiation of progenitor stem cells (PSCs). In addition, SOX9 is the transcription factor responsible for PSCs chondrogenic commitment. To evaluate the effects of the non-amino bisphosphonate clodronate in OA patients we investigated SOX9 gene expression in circulating progenitor cells (CPCs) and in an in vitro OA model. We evaluated pain intensity, mental and physical performance in OA patients, as well as serum biomarkers related to bone metabolism. In addition, in order to improve therapeutic strategies, we assayed nanoparticle-embedded clodronate (NPs-clo) in an in vitro model of chondrogenic differentiation. Our data showed upregulation of SOX9 gene expression upon treatment, suggesting an increase in chondrocytic commitment. Clodronate also reduced osteoarticular pain and improved mental and physical performance in patients. Furthermore, NPs-clo stimulated SOX9 expression more efficaciously than clodronate alone. Clodronate may therefore be considered a good therapeutic tool against OA; its formulation in nanoparticles may represent a promising challenge to counteract cartilage degeneration. View Full-Text
Keywords: clodronate; gene expression; osteoarthritis; progenitor cells; SOX9 clodronate; gene expression; osteoarthritis; progenitor cells; SOX9

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Valenti, M.T.; Mottes, M.; Biotti, A.; Perduca, M.; Pisani, A.; Bovi, M.; Deiana, M.; Cheri, S.; Dalle Carbonare, L. Clodronate as a Therapeutic Strategy against Osteoarthritis. Int. J. Mol. Sci. 2017, 18, 2696.

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