Challenges for Super-Resolution Localization Microscopy and Biomolecular Fluorescent Nano-Probing in Cancer Research
Abstract
:1. Introduction
1.1. Challenges of Cancer Research
1.2. Localization Microscopy
1.3. Nano-Probing for Localization Microscopy
2. Results and Discussion
2.1. Data Interpretation: From Points to Structural Organization
2.2. Cluster Formation of Epidermal Growth Factor Receptors (EGFR) and Their Response to Cancer Treatment
2.3. Chromatin Response after Smad Specific Uboquitin Protein Ligase 2 (Smurf2) Over-Expression and Cell Treatment with the Chemotherapeutic Drug Etoposide
2.4. Chromatin Response to Radiation Treatment Detected by γH2AX Foci Formation and Heterochromatin (HC) Reorganisation
2.5. Chromatin Response to Radiation Treatment Detected by COMBO-FISH of the ALU Consensus Regions
3. Materials and Methods
3.1. Cell Culture and Treatment
3.2. Immunostaining and COMBOinatorial Oligonucleotide Fluorescence In Situ Hybridization (COMBO-FISH)
3.3. Localization Microscopy
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Irradiation Dose (Gy) | Number of Cells Analyzed | Mean Number of Points | Error of the Mean (±) |
---|---|---|---|
control | 80 | 25,839 | 1129 |
0.1 | 83 | 21,669 | 783 |
0.2 | 40 | 25,011 | 1513 |
0.3 | 40 | 17,594 | 1538 |
0.4 | 40 | 19,091 | 1347 |
0.5 | 80 | 17,789 | 676 |
1 | 38 | 13,061 | 518 |
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Hausmann, M.; Ilić, N.; Pilarczyk, G.; Lee, J.-H.; Logeswaran, A.; Borroni, A.P.; Krufczik, M.; Theda, F.; Waltrich, N.; Bestvater, F.; et al. Challenges for Super-Resolution Localization Microscopy and Biomolecular Fluorescent Nano-Probing in Cancer Research. Int. J. Mol. Sci. 2017, 18, 2066. https://doi.org/10.3390/ijms18102066
Hausmann M, Ilić N, Pilarczyk G, Lee J-H, Logeswaran A, Borroni AP, Krufczik M, Theda F, Waltrich N, Bestvater F, et al. Challenges for Super-Resolution Localization Microscopy and Biomolecular Fluorescent Nano-Probing in Cancer Research. International Journal of Molecular Sciences. 2017; 18(10):2066. https://doi.org/10.3390/ijms18102066
Chicago/Turabian StyleHausmann, Michael, Nataša Ilić, Götz Pilarczyk, Jin-Ho Lee, Abiramy Logeswaran, Aurora Paola Borroni, Matthias Krufczik, Franziska Theda, Nadine Waltrich, Felix Bestvater, and et al. 2017. "Challenges for Super-Resolution Localization Microscopy and Biomolecular Fluorescent Nano-Probing in Cancer Research" International Journal of Molecular Sciences 18, no. 10: 2066. https://doi.org/10.3390/ijms18102066