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Int. J. Mol. Sci. 2018, 19(2), 337;

An in Vitro Study on the Effect of Combined Treatment with Photodynamic and Chemical Therapies on Candida albicans

Department of Clinical Pathology, Buddhist Dalin Tzu Chi General Hospital, Chia-Yi 62247, Taiwan
Department of Biomedical Sciences, National Chung Cheng University, Min-Hsiung, Chia-Yi 62102, Taiwan
National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan
Author to whom correspondence should be addressed.
Received: 13 December 2017 / Revised: 15 January 2018 / Accepted: 18 January 2018 / Published: 24 January 2018
(This article belongs to the Special Issue Laser Application in Life Sciences 2018)
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Candida albicans is the most commonly encountered human fungal pathogen, and it is traditionally treated with antimicrobial chemical agents. The antimicrobial effect of these agents is largely weakened by drug resistance and biofilm-associated virulence. Enhancement of the antimicrobial activity of existing agents is needed for effective candidiasis treatment. Our aim was to develop a therapy that combined biofilm disruption with existing antimicrobial agents. Photodynamic therapy (PDT) utilizing curcumin and blue light was tested as an independent therapy and in combination with fluconazole treatment. Viability assays and morphology analysis were used to assess the effectiveness of C. albicans treatment. Results showed that fluconazole treatment decreased the viability of planktonic C. albicans, but the decrease was not as pronounced in adherent C. albicans because its biofilm form was markedly more resistant to the antimicrobiotic. PDT effectively eradicated C. albicans biofilms, and when combined with fluconazole, PDT significantly inhibited C. albicans to a greater extent. This study suggests that the addition of PDT to fluconazole to treat C. albicans infection enhances its effectiveness and can potentially be used clinically. View Full-Text
Keywords: photodynamic therapy; Candida albicans; curcumin; fluconazole; biofilm photodynamic therapy; Candida albicans; curcumin; fluconazole; biofilm

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hsieh, Y.-H.; Zhang, J.-H.; Chuang, W.-C.; Yu, K.-H.; Huang, X.-B.; Lee, Y.-C.; Lee, C.-I. An in Vitro Study on the Effect of Combined Treatment with Photodynamic and Chemical Therapies on Candida albicans. Int. J. Mol. Sci. 2018, 19, 337.

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